Allergen and Epitope Targets of Mouse-Specific T Cell Responses in Allergy and Asthma

Frontiers in Immunology
V SchultenAlessandro Sette

Abstract

Mouse allergy has become increasingly common, mainly affecting laboratory workers and inner-city households. To date, only one major allergen, namely Mus m 1, has been described. We sought to identify T cell targets in mouse allergic patients. PBMC from allergic donors were expanded with either murine urine or epithelial extract and subsequently screened for cytokine production (IL-5 and IFNγ) in response to overlapping peptides spanning the entire Mus m 1 sequence, peptides from various Mus m 1 isoforms [major urinary proteins (MUPs)], peptides from mouse orthologs of known allergens from other mammalian species and peptides from proteins identified by immunoproteomic analysis of IgE/IgG immunoblots of mouse urine and epithelial extracts. This approach let to the identification of 106 non-redundant T cell epitopes derived from 35 antigens. Three major T cell-activating regions were defined in Mus m 1 alone. Moreover, our data show that immunodominant epitopes were largely shared between Mus m 1 and other MUPs even from different species, suggesting that sequence conservation in different allergens is a determinant for immunodominance. We further identified several novel mouse T cell antigens based on their homology to known ma...Continue Reading

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Citations

Jul 18, 2018·Immunology·Sandeep K DhandaAlessandro Sette
May 15, 2018·Frontiers in Immunology·Ricardo da Silva AntunesAlessandro Sette
Jul 3, 2018·Frontiers in Immunology·Sandeep Kumar DhandaAlessandro Sette
Oct 18, 2019·Frontiers in Immunology·Tuomas Virtanen, Marja Rytkönen-Nissinen
Jun 14, 2020·Science Immunology·Grégory SeumoisPandurangan Vijayanand
Oct 2, 2019·Disease-a-month : DM·Roua AzmehRobert D Lloyd
Mar 2, 2021·Frontiers in Immunology·Anna PomésAlessandro Sette

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
biopsies

Software Mentioned

IEDB
restrictor analysis tool for epitopes ( RATE )
FlowJo
MEGA
RATE
Mascot

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