Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity

The Journal of Allergy and Clinical Immunology
Jill GlesnerAnna Pomés

Abstract

Cockroach allergens are an important cause of IgE-mediated sensitization in inner-city asthmatic patients. However, cockroach extracts used for diagnosis and immunotherapy are not standardized. We sought to determine the allergen content of nonstandardized German cockroach extracts and the levels of sensitization to an expanded set of cockroach allergens as determinants of in vitro extract potency for IgE reactivity. Twelve German cockroach extracts were compared for allergen content and potency of IgE reactivity. Bla g 1, Bla g 2, and Bla g 5 were measured by using immunoassays. IgE antibody levels to 8 purified recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9, and 11 were measured by using ImmunoCAP. IgE antibody binding inhibition assays were performed to assess extract in vitro potencies (concentration inhibiting 30% of the total IgE antibody-binding inhibition) relative to an arbitrarily selected reference extract in 5 patients with cockroach allergy. Allergen levels were highly variable. Three new major allergens (groups 6, 9, and 11), were identified among highly cockroach-sensitized subjects (CAP class ≥ 3). Sensitization profiles were unique per subject without immunodominant allergens. The sum of IgE to 8 allerge...Continue Reading

Citations

May 3, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Véronique SchultenAlessandro Sette
Jul 17, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Stefano NebbiaGiovanni Rolla
Aug 27, 2019·Current Opinion in Allergy and Clinical Immunology·Andreas BonertzStefan Vieths
Jul 17, 2019·Current Protein & Peptide Science·Kyoung Yong Jeong, Jung-Won Park
Aug 1, 2019·Current Protein & Peptide Science·Nitat SookrungWanpen Chaicumpa
May 22, 2020·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Montserrat Alvaro-LozanoMarta Vazquez-Ortiz
Mar 19, 2020·Bioscience Reports·Hiu Yan LamKwang Seok Ahn
Mar 21, 2019·Frontiers in Immunology·Giovanni BirruetaVéronique Schulten
Jul 4, 2019·International Journal for Parasitology·Fan Zhang, Rongai Yang
Oct 2, 2019·Disease-a-month : DM·Roua AzmehRobert D Lloyd
Apr 4, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Samuel T MindayeJay E Slater
Mar 2, 2021·Frontiers in Immunology·Anna PomésAlessandro Sette
Sep 4, 2020·Allergy·Öykü ÜzülmezHeimo Breiteneder
May 7, 2020·The World Allergy Organization Journal·Luis CaraballoAlexander Karaulov
Apr 19, 2021·Allergy·Maksymilian ChruszczHeimo Breiteneder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Related Papers

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
Taruna KhuranaJay E Slater
Current Allergy and Asthma Reports
L K ArrudaAnna Pomés
The Journal of Allergy and Clinical Immunology
Ginger L ChewJudith S Jacobson
International Archives of Allergy and Immunology
Kyoung Yong JeongTai-Soon Yong
© 2022 Meta ULC. All rights reserved