Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin

European Journal of Immunology
L T BarataA B Kay

Abstract

We have attempted to identify Fc epsilon RI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the alpha, beta, and gamma chains of Fc epsilon RI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-alpha chain antibody, the Fc epsilon RI alpha protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of Fc epsilon RI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express Fc epsilon. RI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.

References

Oct 1, 1991·The Journal of Allergy and Clinical Immunology·D H BroideS I Wasserman
Jun 1, 1995·The Journal of Clinical Investigation·M KanekoH Kita
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L K ErnstC L Anderson
Dec 1, 1995·The Journal of Allergy and Clinical Immunology·N TeradaY Tanaka
Jan 1, 1995·Advances in Immunology·A J WardlawA B Kay
Jun 1, 1996·American Journal of Respiratory and Critical Care Medicine·M HumbertA B Kay
Jan 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L Taborda-BarataA B Kay

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Citations

Sep 18, 2007·Journal of Pediatric Gastroenterology and Nutrition·Chris A LiacourasGlenn T Furuta
Oct 23, 2019·Frontiers in Immunology·Kelly N MessinghamJanet A Fairley
Aug 24, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F Purello-D'AmbrosioG F Bagnato
Sep 2, 1998·The Journal of Allergy and Clinical Immunology·R A TahaQ Hamid

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