PMID: 7543502Aug 1, 1995Paper

Allergen-specific IgE production of committed B cells from allergic patients in vitro

The Journal of Allergy and Clinical Immunology
P SteinbergerR Valenta

Abstract

The allergen-specific in vitro IgE synthesis in blood leukocytes from patients with allergy was monitored outside the pollen season with recombinant and natural pollen allergens and was compared with the total IgE production. The addition of interleukin-4 (IL-4) and antibody to CD40 increased the amount of total IgE by up to 20-fold in the culture supernatants of peripheral blood leukocytes from patients with allergy that could be antagonized by a neutralizing anti-IL-4 antibody in a dose-dependent manner. In contrast to total IgE, the amount of allergen-specific IgE was not affected by IL-4, and anti-CD40 or anti-IL-4, treatment. With oligonucleotides specific for IgE, complementary DNA from the amino terminal of the IgE heavy chain could be reversely transcribed and amplified by polymerase chain reaction from RNA of patients' unstimulated blood leukocytes, indicating that the IgE secretion in the cultures is due to a de novo IgE synthesis. It is concluded that the peripheral blood of patients with allergy contains long-lived allergen-specific B cells, which are not responsive to IL-4-mediated signals. These results may have implications for attempts to modulate specific IgE production in allergic patients with cytokines or cy...Continue Reading

Citations

Apr 16, 2013·Current Allergy and Asthma Reports·Julia Eckl-Dorna, Verena Niederberger
Dec 1, 1995·Current Opinion in Immunology·R Valenta, D Kraft
Aug 5, 1998·Allergy·R ValentaD Kraft
Dec 16, 2011·The Journal of Allergy and Clinical Immunology·Melissa DullaersBart N Lambrecht
Aug 29, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J Eckl-DornaV Niederberger
Jan 1, 1997·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·C Y ZhouR G Townley
Mar 20, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Nobuharu Ohshima, Shunsei Hirohata
Oct 24, 2006·International Archives of Allergy and Immunology·Verena NiederbergerRudolf Valenta
Nov 9, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y J ChoH B Moon
Jul 3, 2002·Nature Reviews. Immunology·Rudolf Valenta
Mar 15, 2014·Nature Reviews. Immunology·Lawren C Wu, Ali A Zarrin
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christian LupinekRudolf Valenta
Dec 7, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Kelly M CoxLoren D Erickson
Aug 31, 2019·Cells·Julia Eckl-DornaRudolf Valenta
Feb 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christoph MadritschSabine Flicker
Feb 15, 2001·The Journal of Allergy and Clinical Immunology·A Bufe

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