PMID: 9542607May 23, 1998Paper

Allergen-specific increase in interleukin (IL)-4 and IL-5 secretion from peripheral blood mononuclear cells during birch-pollen immunotherapy

Allergy
R MovérareL Elfman

Abstract

The mechanisms behind the effects of immunotherapy (IT) with birch-pollen extract are largely unknown. In this pilot study, we measured the cytokine secretion in vitro from peripheral blood mononuclear cells (PBMC) obtained from birch-pollen-allergic patients undergoing IT treatment (n = 4) or placebo administration (n = 4), collected before treatment, 1 and 4 weeks after start of treatment, and during and just after the pollen season (12-14 weeks after start of treatment). The PBMC were stimulated with birch-pollen extract in vitro for 7 days, followed by restimulation with the mitogens phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) for 24 h, to enhance the production of cytokines. The supernatants were analyzed with ELISA and radioimmunoassay for interferon-gamma (IFN-gamma), interleukin (IL)-4, and IL-5. In the therapy group, we noted an increased secretion of IL-4 and IL-5 from PBMC collected at 4 weeks after the start of treatment (IL-4: 29 +/- 21 pg/ml [day 0] to 374 +/- 448 pg/ml [week 4], mean +/- SD; IL-5: 95 +/- 48 pg/ml to 1147 +/- 697 pg/ml). No increase was seen in the placebo group. During the pollen season, we noted a trend toward increased IL-4 and IL-5 secretion in both groups. We conclude t...Continue Reading

References

May 1, 1976·The Journal of Clinical Investigation·R EvansR E Rocklin
Dec 1, 1992·Immunology Today·C J Corrigan, A B Kay
Jul 11, 1992·The Medical Clinics of North America·J L Ohman
Jan 1, 1991·International Journal of Clinical & Laboratory Research·S Romagnani
Jan 1, 1991·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S R DurhamA B Kay
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PèneJ Banchereau
Oct 1, 1986·The Journal of Allergy and Clinical Immunology·M J ReidB L Nelson
Feb 1, 1973·The Journal of Clinical Investigation·L M LichtensteinB M Hill
Jan 1, 1973·International Archives of Allergy and Applied Immunology·S RomagnaniM Ricci
May 1, 1995·International Archives of Allergy and Immunology·C EbnerD Kraft
Dec 1, 1993·The Journal of Experimental Medicine·H SecristD T Umetsu
Sep 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M McHughP W Ewan
Nov 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M JutelW J Pichler
Nov 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M Tengvall LinderA Scheynius

❮ Previous
Next ❯

Citations

Oct 28, 1999·Allergy·M AbramsonJ Weiner
Jul 30, 2002·Allergy·J M Hopkin
Apr 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T Jung
Aug 10, 2000·Scandinavian Journal of Immunology·R MovérareG Stâlenheim
Jan 27, 2007·The Cochrane Database of Systematic Reviews·M A CalderonS Durham
Aug 6, 2010·The Cochrane Database of Systematic Reviews·Michael J AbramsonJohn M Weiner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.