Allergenic Characterization of 27-kDa Glycoprotein, a Novel Heat Stable Allergen, from the Pupa of Silkworm, Bombyx mori

Journal of Korean Medical Science
Kyoung Yong JeongJung Won Park

Abstract

Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase, has been identified. The purpose of this study was to identify novel food allergens in silkworm pupa by analyzing a protein extract after heat treatment. Heat treated extracts were examined by proteomic analysis. A 27-kDa glycoprotein was identified, expressed in Escherichia coli, and purified. IgE reactivity of the recombinant protein was investigated by ELISA. High molecular weight proteins (above 100 kDa) elicited increased IgE binding after heat treatment compared to that before heat treatment. The molecular identities of these proteins, however, could not be determined. IgE reactivity toward a 27-kDa glycoprotein was also increased after heating the protein extract. The recombinant protein was recognized by IgE antibodies from allergic subjects (33.3%). Glycation or aggregation of protein by heating may create new IgE binding epitopes. Heat stable allergens are shown to be important in silkworm allergy. Sensitization to the 27-kDa glycoprotein from silkworm may contribute to elevation of IgE to silkworm.

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Citations

Jun 28, 2017·Molecular Nutrition & Food Research·José Carlos RibeiroJoão Fonseca
Feb 23, 2019·Archives of Insect Biochemistry and Physiology·Xiao-Dong LingXing-Xu Zhao
Jul 17, 2019·Current Protein & Peptide Science·Kyoung Yong Jeong, Jung-Won Park
Mar 25, 2017·Molecular Medicine Reports·Kyoung Yong JeongJung-Won Park
Jun 23, 2020·Food Science & Nutrition·Alessandra Anna AltomareAlfonsina D'Amato
Apr 15, 2020·Allergologia et immunopathologia·Laura M L AraujoNelson A Rosário Filho
Jan 8, 2021·Food Chemistry·Bei ZhengPeng Chen
May 8, 2018·Molecular Immunology·Steffie de Gier, Kitty Verhoeckx
Jun 1, 2021·Current Allergy and Asthma Reports·Laura De MarchiGianni Zoccatelli
Aug 18, 2021·Food Science & Nutrition·Xuli WuZhigang Liu
Sep 21, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yanlin LongLili Chen

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Methods Mentioned

BETA
ELISA
chemical modification

Software Mentioned

Mascot

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