Allergenic epitopes of ovalbumin (OVA) in patients with hen's egg allergy: inhibition of basophil histamine release by haptenic ovalbumin peptide

Clinical and Experimental Immunology
K HonmaH Niimi

Abstract

We studied allergenic determinants that induce hypersensitivity to OVA, the major allergen in egg allergy, using immunoblot and histamine release assays. Immunoblot analysis demonstrated a part of the OVA epitope was in the C-terminal region comprising residues 347-385 (OVA347-385). Histamine was released from basophils of a patient with egg allergy upon stimulation with the OVA fragment corresponding to OVA347-385. Furthermore, detailed epitope mapping using overlapping peptides (residues 347-366, OVA-A; residues 357-376, OVA-B; and residues 367-385, OVA-C) in the OVA 347-385 region was carried out using the histamine release assay. In order for histamine release from basophils to occur, the allergen must possess two or more allergenic determinants located on the protein molecule at distances that would be equivalent to the distances between IgE molecules on the membrane surface. these results suggest that there are at least two epitopes that bind IgE antibodies on each OVA peptide. In addition, one epitope that binds IgE antibodies in two patients appears to reside in the haptenic peptide OVA357-366 (OVA-B1). The histamine release from basophils stimulated by OVA-B was completely inhibited by OVA-B1 in one of these patients. ...Continue Reading

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Citations

Dec 22, 1999·Clinical Reviews in Allergy & Immunology·J S Stanley, G A Bannon
Jan 20, 2015·Food & Function·S BenedéR López-Fandiño
Mar 29, 2006·Current Allergy and Asthma Reports·Ralf G HeineDavid J Hill
Jun 12, 2008·Journal of Agricultural and Food Chemistry·Yoshinori Mine, Marie Yang
Dec 5, 2008·Journal of Agricultural and Food Chemistry·Ivan López-ExpósitoRosina López-Fandiño
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·R Bredehorst, K David
May 10, 2003·Journal of Immunological Methods·Wei-Jen TsaiChiou-Ying Yang
Sep 21, 2021·Journal of Theoretical Biology·Stephen LindslyIndika Rajapakse

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