Abstract
We studied allergenic determinants that induce hypersensitivity to OVA, the major allergen in egg allergy, using immunoblot and histamine release assays. Immunoblot analysis demonstrated a part of the OVA epitope was in the C-terminal region comprising residues 347-385 (OVA347-385). Histamine was released from basophils of a patient with egg allergy upon stimulation with the OVA fragment corresponding to OVA347-385. Furthermore, detailed epitope mapping using overlapping peptides (residues 347-366, OVA-A; residues 357-376, OVA-B; and residues 367-385, OVA-C) in the OVA 347-385 region was carried out using the histamine release assay. In order for histamine release from basophils to occur, the allergen must possess two or more allergenic determinants located on the protein molecule at distances that would be equivalent to the distances between IgE molecules on the membrane surface. these results suggest that there are at least two epitopes that bind IgE antibodies on each OVA peptide. In addition, one epitope that binds IgE antibodies in two patients appears to reside in the haptenic peptide OVA357-366 (OVA-B1). The histamine release from basophils stimulated by OVA-B was completely inhibited by OVA-B1 in one of these patients. ...Continue Reading
References
Feb 1, 1978·Allergy·K Aas
Feb 1, 1992·Molecular Immunology·J Le MaoB David
Oct 1, 1992·Molecular Immunology·H KahlertM Schlaak
Sep 1, 1990·Molecular Immunology·G Johnsen, S Elsayed
Sep 6, 1990·Nature·P E SteinR W Carrell
Apr 1, 1988·The Journal of Clinical Endocrinology and Metabolism·N ShimojoH Nakajima
May 1, 1988·Scandinavian Journal of Immunology·S ElsayedM B Haugstad
Nov 1, 1988·The Journal of Clinical Endocrinology and Metabolism·Y KohnoT Hosoya
Aug 15, 1970·Nature·U K Laemmli
Aug 1, 1965·Biochemistry·S F SchlossmanH A Sober
Sep 1, 1966·Immunochemistry·H Van VunakisL Levine
May 1, 1983·The Journal of Allergy and Clinical Immunology·H A Sampson
Jan 1, 1994·International Archives of Allergy and Immunology·K HonmaH Niimi
Jul 1, 1993·Allergy Proceedings : the Official Journal of Regional and State Allergy Societies·L M Du Buske
May 1, 1994·International Archives of Allergy and Immunology·S Elsayed, L Stavseng
Jun 1, 1994·The Journal of Allergy and Clinical Immunology·J Bernhisel-BroadbentH A Sampson
Citations
Jul 14, 2020·Food & Function·Behzad GazmeChibuike C Udenigwe
Aug 1, 1997·Allergy·R van Ree
Mar 22, 2002·Allergy·T-W NgS L Prescott
Dec 22, 1999·Clinical Reviews in Allergy & Immunology·J S Stanley, G A Bannon
Dec 15, 2006·Plant Biotechnology Journal·Gijs A Kleter, Ad A C M Peijnenburg
Dec 7, 2013·Immunology·Shin YoshinoChutha Sae-Wong
Jan 20, 2015·Food & Function·S BenedéR López-Fandiño
Jun 20, 2001·Immunology·S ToyabeM Uchiyama
Mar 29, 2006·Current Allergy and Asthma Reports·Ralf G HeineDavid J Hill
Feb 2, 2021·Frontiers in Pharmacology·Taizhen LiangLin Li
Jun 12, 2008·Journal of Agricultural and Food Chemistry·Yoshinori Mine, Marie Yang
Dec 5, 2008·Journal of Agricultural and Food Chemistry·Ivan López-ExpósitoRosina López-Fandiño
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·R Bredehorst, K David
May 10, 2003·Journal of Immunological Methods·Wei-Jen TsaiChiou-Ying Yang
Dec 20, 2013·Journal of Agricultural and Food Chemistry·Sara BenedéElena Molina
Apr 28, 2010·Retrovirology·Lin LiShuwen Liu
Sep 21, 2021·Journal of Theoretical Biology·Stephen LindslyIndika Rajapakse