Allergenicity assessment of transgenic mustard (Brassica juncea) expressing bacterial codA gene

Allergy
A K SinghNaveen Arora

Abstract

Assessing the allergenicity and toxicity of genetically modified (GM) crops is essential before they become a regular part of our food supply. The present study aimed to assess the allergenicity of Brassica juncea (mustard) expressing choline oxidase (codA) gene from Arthrobacter globiformis that provides resistance against abiotic stresses. SDAP, Farrp, and Swiss-Prot databases were used to study allergenicity of choline oxidase. Digestibility of choline oxidase was assessed in simulated gastric fluid (SGF). Specific immunoglobulin E (IgE) reactivity of native and GM mustard was compared by using enzyme-linked immunosorbent assay (ELISA) and skin tests in respiratory-allergic patients. Allergenicity of GM and native mustard proteins was compared in Balb/c mice. Choline oxidase showed no significant homology with allergenic proteins in SDAP and Farrp databases. Cross-reactive epitope search showed a stretch similar to Hev b 6 having some antigenic properties. Purified choline oxidase showed complete degradation with SGF. Skin prick test of native and GM mustard extract on respiratory allergic patients showed significant correlation (P < 0.05). ELISA with 96 patients' sera showed comparable IgE reactivity. Balb/c mice immunized ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1991·International Archives of Allergy and Applied Immunology·H D ShenS H Han
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Jan 1, 1980·Transactions of the Royal Society of Tropical Medicine and Hygiene·J E Sippel, A Voller
Dec 1, 1995·The Journal of Allergy and Clinical Immunology·A W Burks, R L Fuchs
Mar 14, 1996·The New England Journal of Medicine·J A NordleeR K Bush
Oct 1, 1996·Nature Biotechnology·J D AstwoodR L Fuchs
Oct 20, 1999·Methods : a Companion to Methods in Enzymology·L M KnippelsA H Penninks
Dec 10, 1999·Nature·P G HoltP D Sly
Jun 10, 2000·The Plant Journal : for Cell and Molecular Biology·A SakamotoN Murata
Jul 11, 2000·The Journal of Allergy and Clinical Immunology·X M LiH A Sampson
Nov 14, 2000·Plant Science : an International Journal of Experimental Plant Biology·K V Prasad Pardha Saradhi P
Jan 9, 2001·International Archives of Allergy and Immunology·T A BeardsleeJ P Markwell
Apr 27, 2001·Toxicology Letters·R J Dearman, I Kimber
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·H A KuiperE J Kok
Jan 10, 2002·Environmental Health Perspectives·Luca Bucchini, Lynn R Goldman
Sep 10, 2002·International Archives of Allergy and Immunology·Ronald E HilemanSusan L Hefle
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Piia KarisolaHarri Alenius
Apr 6, 2004·Plant Physiology and Biochemistry : PPB·Daniela ParvanovaDimitar Djilianov
May 8, 2004·The Journal of Allergy and Clinical Immunology·Hugh A Sampson
Oct 27, 2004·The Plant Journal : for Cell and Molecular Biology·Eung-Jun ParkTony H H Chen
Mar 1, 2005·Vaccine·Carol O Tacket
Aug 9, 2005·The Journal of Allergy and Clinical Immunology·Rita BatistaMaria Margarida Oliveira

❮ Previous
Next ❯

Citations

May 20, 2008·Analytical and Bioanalytical Chemistry·Stephanie Bachas-Daunert, Sapna K Deo
Jun 18, 2009·Journal of Clinical Immunology·Deepsikha SrivastavaShailendra Nath Gaur
Oct 4, 2007·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Deepsikha SrivastavaShailendra N Gaur
Aug 16, 2011·Journal of Agricultural and Food Chemistry·Prerna SharmaNaveen Arora
Nov 8, 2008·Critical Reviews in Food Science and Nutrition·Artemis Dona, Ioannis S Arvanitoyannis
Oct 13, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Bryan Delaney
Sep 13, 2008·Free Radical Biology & Medicine·Prabhanshu TripathiNaveen Arora
Feb 6, 2007·Immunology and Allergy Clinics of North America·Catherine H ScheinWerner Braun
Apr 29, 2009·European Journal of Clinical Investigation·V T SudhaB P Singh
Jul 31, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ramkrashan KaseraNaveen Arora
Apr 10, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Zhenxing LiLimin Zhang
Mar 1, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ankita MishraNaveen Arora
Nov 22, 2017·Clinical Reviews in Allergy & Immunology·Akanksha SharmaPremendra D Dwivedi
Jun 29, 2006·World Journal of Gastroenterology : WJG·De-Ying TianNing-Shao Xia
Dec 15, 2016·Asthma Research and Practice·Alessandro FiocchiVincenzo Fierro
Apr 20, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Mahmudur RahmanBronwyn J Barkla
Nov 28, 2008·Journal of Agricultural and Food Chemistry·Abinav K SinghNaveen Arora
Jul 30, 2018·International Archives of Allergy and Immunology·Kashinath BhattacharyaSwati Gupta Bhattacharya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.