Allergenicity of sigma and delta class glutathione S-transferases from the German cockroach

International Archives of Allergy and Immunology
Kyoung Yong JeongTai-Soon Yong

Abstract

Cockroach glutathione S-transferases (GSTs) are known to elicit strong IgE responses. This study was undertaken to compare the IgE reactivity of German cockroach GSTs, Bla g 5 (sigma class) and delta class GST (BgGSTD1). Full-length Bla g 5 and BgGSTD1 were cloned, and their recombinant proteins were expressed and purified. Their IgE reactivities and cross-reactivities were examined by ELISA using sera from cockroach-sensitized subjects. A predominant variant of Bla g 5 cDNA has amino acid substitutions at positions 10 (C to F) and 42 (N to K). BgGSTD1 has substitutions at positions 27 (E to N) and 207 (K to R). Sera from cockroach-sensitized patients showed 20.5% IgE reactivity to Bla g 5 and 17.9% IgE reactivity to BgGSTD1. However, inhibition studies using 1 serum sample with the highest IgE reactivity showed limited cross-reactivity. IgE-binding frequency to the cockroach GSTs was low, but the titer of IgE reactivity was strong in some sera. The inclusion of different classes of GSTs could be helpful for the delicate diagnosis and immunotherapy of cockroach allergy.

Citations

Apr 8, 2014·Mediators of Inflammation·Ji-Fu WeiShaoheng He
May 1, 2012·The Journal of Allergy and Clinical Immunology·Helton C SantiagoThomas B Nutman
May 2, 2015·The Journal of Allergy and Clinical Immunology·Geoffrey A MuellerAnna Pomés
Aug 31, 2016·The American Journal of Tropical Medicine and Hygiene·Helton C Santiago, Thomas B Nutman
Mar 10, 2015·Allergy, Asthma & Immunology Research·Kyoung Yong JeongJung Won Park
Nov 2, 2012·Allergy, Asthma & Immunology Research·Kyoung Yong JeongChein-Soo Hong
Sep 6, 2012·Allergy, Asthma & Immunology Research·Myung Hyun Sohn, Kyu-Earn Kim
Nov 4, 2009·The Korean Journal of Parasitology·Tai-Soon Yong, Kyoung Yong Jeong
Jul 17, 2019·Current Protein & Peptide Science·Kyoung Yong Jeong, Jung-Won Park
Aug 1, 2019·Current Protein & Peptide Science·Nitat SookrungWanpen Chaicumpa

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