PMID: 3989145May 1, 1985Paper

Allergens in Hymenoptera venom XIII: Isolation and purification of protein components from three species of vespid venoms

The Journal of Allergy and Clinical Immunology
D R Hoffman

Abstract

Pure venoms were collected from individual insects of the species Dolichovespula maculata, white-faced hornet, Vespula squamosa, southern yellow jacket, and Polistes exclamans, paper wasp (one species). The venoms were first fractionated by high-resolution gel filtration on a 1.6 m column of Sephadex G-75 superfine, and the components were then purified by high-performance, ion-exchange chromatography on a Mono-S cation exchange column followed by a further gel filtration step. The isolated components were evaluated for purity by sodium dodecyl sulfate polyacrylamide gel electrophoresis by use of two different types of silver stains, by assays for enzyme activities, and by immunodiffusion with the use of rabbit antisera. The protein components were isolated in highly purified states by these techniques. Only three significant proteins were found in V. squamous venom: phospholipase (PL) A and B, hyaluronidase (HYAL), and antigen 5 (Ag 5). D. maculata venom contained HYAL, Ag 5, two isozymes of PL A and B, a high-molecular-weight protein, and several trace proteins. No significant amounts of proteases were found in D. maculata venom. P. exclamans venom contained HYAL, PL A and B, Ag 5, a high-molecular-weight protein, and several...Continue Reading

Citations

Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·J O SchmidtW L Overal
Nov 1, 1988·The Journal of Allergy and Clinical Immunology·D R HoffmanC T Stafford
Oct 24, 2003·Biochimica Et Biophysica Acta·Barbara PanteraLuigia Pazzagli
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·M R de Oliveira, M S Palma
Apr 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·H Costa, M S Palma
Oct 1, 1996·Current Opinion in Genetics & Development·J M Hawdon, P J Hotez
Mar 22, 2016·Journal of Pharmacopuncture·Hossein ZolfagharianMahdi Babaie
Nov 24, 1999·The Veterinary Clinics of North America. Small Animal Practice·D E Bevier
Sep 2, 2008·Journal of Molecular Biology·Sivaraman PadavattanZora Marković-Housley

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