Allergic drug reactions: an in vitro model using a mixed function oxidase complex to demonstrate antibodies with specificity for a practolol metabolite

Clinical Allergy
H E AmosH A Atkinson

Abstract

Reactive metabolite products of the drug practolol were generated in vitro using the rat liver mixed function oxidase complex. The metabolites were spontaneously coupled to a non-agglutinating rabbit antibody to human O red blood cells, so forming a metabolite-antibody reagent. This reagent was then used to passively sensitize human O red cells which subsequently acted as indicator cells for detecting anti-metabolite antibodies.

Citations

Oct 1, 1987·Agents and Actions·G R ElliottJ W Bridges
Jan 1, 1979·International Journal of Immunopharmacology·H E Amos
Jun 1, 1978·British Journal of Pharmacology·A Gibson, M Samini

Related Concepts

Antibody Specificity
Autoantibodies
Drug Allergy
Medicinal Plants Testing, Preclinical
Hemagglutination Test
Mixed Function Oxygenases
Microsomes, Liver
Oxidase
Dalzic

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