Allogeneic adipose-derived mesenchymal stem cell sheet that produces neurological improvement with angiogenesis and neurogenesis in a rat stroke model

Journal of Neurosurgery
Bikei RyuTatsuya Shimizu

Abstract

OBJECTIVEStem cell therapy is a promising strategy for the treatment of severe cerebral ischemia. However, targeting sufficient grafted cells to the affected area remains challenging. Choosing an adequate transplantation method for the CNS appears crucial for this therapy to become a clinical reality. The authors used a scaffold-free cell sheet as a translational intervention. This method involves the use of cell sheet layers and allows the transplantation of a large number of cells, locally and noninvasively. The authors evaluated the effectiveness of allogeneic adipose tissue-derived mesenchymal stem cell sheets in a rat model of stroke.METHODSThe animals, subjected to middle cerebral artery occlusion, were randomly divided in two groups: one in which a cell sheet was transplanted and the other in which a vehicle was used (n = 10/group). Over a period of 14 days after transplantation, the animals' behavior was evaluated, after which brain tissue samples were removed and fixed, and the extent of angiogenesis and infarct areas was evaluated histologically.RESULTSCompared to the vehicle group, in the cell sheet group functional angiogenesis and neurogenesis were significantly increased, which resulted in behavioral improvement. ...Continue Reading

References

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Citations

Nov 25, 2020·Emerging Topics in Life Sciences·Makoto KondoTeruo Okano
Jan 4, 2021·CNS Neuroscience & Therapeutics·Liyun WangJian Shen
Oct 16, 2020·Journal of Stroke·Shuai ZhangXiaofeng Jia
May 4, 2021·Frontiers in Cellular Neuroscience·Xi-Le ZhangGuo-Qing Zheng
May 14, 2021·Translational Stroke Research·Yiyang LiYonghua Zhao
Aug 28, 2021·International Journal of Molecular Sciences·Sunhong MoonYoon Kyung Choi

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