PMID: 6965796Feb 1, 1980Paper

Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions

Proceedings of the National Academy of Sciences of the United States of America
Y ReisnerR A Good

Abstract

Mouse spleen cells sequentially agglutinated by soybean agglutinin (SBA) and peanut agglutinin (PNA) previously had been shown to be sufficiently depleted of graft-versus-host activity to allow reconstitution of lethally irradiated allogeneic recipient mice. We have now tested the extent of T-cell depletion in this cell fraction by various in vitro assays, including cytotoxicity testing with anti-Thy-1 antiserum, mitogenic response to the T-cell mitogens concanavalin A and phytohemagglutinin and allogeneic responsiveness in the mixed lymphocytes culture assay. By these criteria the SBA+, PNA+ spleen fraction, used previously in the in vivo experiments, was found to possess about 1% T-cell contamination. The slight contamination with T cells previously found in the singly agglutinated SBA fraction can be removed by a second fractionation with SBA, thus eliminating the possibility that a minor T-cell subpopulation bears receptors for SBA. Finally, we demonstrated that the twice-agglutinated fraction, by SBA and PNA or by SBA alone, contains a significant number of prothymocytes, thereby indicating that mouse prothymocytes bear receptors for both SBA and PNA. The implication of these findings to bone marrow transplantation in huma...Continue Reading

References

Oct 18, 1976·Biochemical and Biophysical Research Communications·Y ReisnerN Sharon
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y ReisnerN Sharon
Nov 1, 1977·Cellular Immunology·A EisenthalM Feldman
Oct 1, 1979·Cellular Immunology·H RabinovichA Globerson
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y ReisnerB Ramot
May 1, 1978·Cellular Immunology·N Haran-GheraG Goldstein
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg

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Citations

Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y ReisnerN Haran-Ghera
Jan 1, 1991·American Journal of Obstetrics and Gynecology·T M CrombleholmeE D Zanjani
Jan 18, 2011·Hematology/oncology Clinics of North America·David Hagin, Yair Reisner
Feb 2, 2010·Immunology and Allergy Clinics of North America·David Hagin, Yair Reisner
Jan 1, 1981·Microbiology and Immunology·K S AkagawaT Tokunaga
Jan 1, 1984·Critical Reviews in Oncology/hematology·H E Broxmeyer
Jun 19, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Feifei WangYusuke Sagara
Feb 1, 1985·European Journal of Immunology·S MoreckiS Slavin
Mar 15, 1982·International Journal of Cancer. Journal International Du Cancer·M J BrundaH T Holden
Mar 1, 1982·Hospital Practice·R A Good

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