Allogeneic mesenchymal stem cells and growth factors in gel scaffold repair osteochondral defect in rabbit.

Regenerative Medicine
Mudasir Bashir GugjooGutulla Taru Sharma

Abstract

Aim: An attempt was made to improve osteochondral healing with allogeneic mesenchymal stem cells (MSCs) along with certain growth factors. Materials & methods: Induced knee osteochondral defects were filled as: phosphate buffer saline (group A); MSCs in collagen gel (group B); group B plus insulin like growth factor-1 (group C); group C plus transforming growth factor β-1 (group D). Results: Gross and scanning electron microscopy showed superior morphology and surface architecture of the healed tissue in groups D and C. Histologically, group D revealed hyaline cartilage characteristic features followed in order by group C and group B. In all treatment groups, chondrogenic matrix, collagen II2B (col II 2B) and aggrecan were secreted. Conclusion: Combined use of MSCs and growth factors could accelerate osteochondral healing.

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Methods Mentioned

BETA
lavage
density gradient centrifugation
scanning electron microscopy
Reverse Transcription
PCR
electrophoresis

Software Mentioned

ImageJ
SPSS
ImagJ

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