Allogenic vs. synthetic granules for bone tissue engineering: an in vitro study

Progress in Biomaterials
Farnaz KouhestaniSaeed Reza Motamedian

Abstract

The aim of this study was to compare human dental pulp stem cells' (DPSCs) attachment, proliferation and osteogenic differentiation on allogenic and synthetic biphasic bone granules. In this in vitro study, two types of bone granules were used: allograft [freeze-dried bone allograft (FDBA)] and biphasic granules [hydroxyapatite/beta-tricalcium phosphate (HA/β-TCP)]. By isolation of DPSCs, their attachment to bone granules was observed by scanning electron microscope (SEM) at day 1 and 7 of cultivation. Vital cells were measured by MTT assay at 1, 3, and 7 days of cell culture. Comparison of vital cells at different time points was considered as cell proliferation. Finally, differentiation of DPSCs was evaluated by measurement of alkaline phosphatase (ALP) activity 3, 7, 14, and 21 days after cell seeding in standard and osteogenic media. Data were analyzed using two-way ANOVA with a significant level of 0.05. Attachment of DPSCs on FDBA granules seemed relatively stronger. The number of cells (based on MTT values) and ALP activity of the cells cultured on both study groups increased between time points (p ≤ 0.001). FDBA granules had more cells compared to HA/β-TCP granules (p < 0.001). There was no significant difference betwee...Continue Reading

References

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Citations

Feb 2, 2021·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Onur KoçFigen Fevziye Kaymaz
May 1, 2021·International Journal of Environmental Research and Public Health·Julia Guerrero-GironésMiguel R Pecci-Lloret

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Methods Mentioned

BETA
flow cytometry
scanning electron microscopy
xenograft

Software Mentioned

SPSS
ImageJ

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