Allograft acceptance and rejection, mediated by a liver suppressor factor, LSF-1, purified from serum of liver transplanted rats

Transplant Immunology
C Edwards-SmithN Kamada

Abstract

In certain rat strain combinations liver allografts are spontaneously accepted without immunosuppression and induce donor-specific tolerance to further skin and heart graft in the recipient. Such an effect is also transferrable using serum from orthotopically liver transplanted rats (OLT serum). In the OLT serum of one such combination, DA (RT1a) donor into PVG (RT1c) recipient, a 40 kDa protein (liver suppressor factor, LSF-1) has been identified and shown to be immunosuppressive in vitro. The aim of the present study is to investigate the immunological effect of LSF-1 and a polyclonal antibody (anti-LSF-1) against this molecule, in a rat heterotopic heart transplant (HHT) model and OLT model, respectively. Intramuscular injection of 300 micrograms of LSF-1, 1 h postoperatively, into a PVG recipient of either a DA or BN (RT1n) cardiac allograft caused significant prolongation of graft survival. Intravenous injection of polyclonal rabbit sera raised against an N-terminal peptide of LSF-1 (anti-LSF-1), within 1 h postoperatively, had variable effects on the survival of DA liver grafts in PVG recipients. In 5/6 cases injection of between 1 and 2 ml of anti-LSF-1 resulted in death of the recipient. Histological examination of the ...Continue Reading

References

Jul 1, 1990·Electrophoresis·T Choli, B Wittmann-Liebold
Sep 13, 1984·The New England Journal of Medicine·B D MyersM Perlroth
Aug 27, 1981·Nature·N KamadaB Roser
Dec 1, 1994·Transplant Immunology·A W Thomson
Jan 8, 1994·Lancet·R Calne, H Davies

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Citations

Mar 20, 1999·Transplantation Proceedings·S GotoC L Chen
Sep 30, 2005·Annals of Surgery·Andreas G TzakisPhillip Ruiz
May 22, 2013·Clinical & Developmental Immunology·Toshiaki NakanoShigeru Goto
Sep 27, 2000·Transplant Immunology·K C ChiangT Hashimoto
Jan 1, 2003·Transplant Immunology·Frank VariShigeru Goto
Feb 20, 2007·Transplant Immunology·Toshiaki NakanoYu-Fan Cheng
Jan 17, 2015·Scandinavian Journal of Gastroenterology·Jonas VarkeyGustaf Herlenius
Jan 24, 2015·Current Gastroenterology Reports·Chandrashekhar A KubalA Joseph Tector
Jun 28, 2014·Journal of Leukocyte Biology·Sven BurghardtGisa Tiegs
Mar 19, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Olivia M Martinez, Hugo R Rosen
Jan 27, 2006·Transplantation·Hidenori TakahashiPhillip Ruiz

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