Allograft Inflammatory Factor-1 Governs Hematopoietic Stem Cell Differentiation Into cDC1 and Monocyte-Derived Dendritic Cells Through IRF8 and RelB in vitro

Frontiers in Immunology
Diana M ElizondoMichael W Lipscomb

Abstract

The multistep differentiation process from hematopoietic stem cells through common myeloid progenitors into committed dendritic cell (DC) subsets remains to be fully addressed. These studies now show that Allograft Inflammatory Factor-1 (AIF1) is required for differentiation of classical DC type 1 (cDC1) subsets and monocyte-derived DC (Mo-DC). Phenotypic studies found that AIF1 expression increased in committed subsets differentiating from common myeloid progenitors (CMP). However, silencing AIF1 expression in hematopoietic stem progenitors restrained the capacity to differentiate into Mo-DC and cDC1 cell subsets under GM-CSF or Flt3-L stimuli conditions, respectively. This was further marked by restrained expression of IRF8, which is critical for development of Mo-DC and cDC1 subsets. As a result, absence of AIF1 restrained the cells at the Lin-CD117+FcγR-CD34+ CMP stage. Further biochemical studies revealed that abrogating AIF1 resulted in inhibition of the NFκB family member RelB expression and p38 MAPK phosphorylation during differentiation of Mo-DC. Lastly, protein binding studies identified that AIF1 interacts with protein kinase C (PKC) to influence downstream signaling pathways. Taken together, this is the first report...Continue Reading

References

Nov 1, 2001·The Journal of Biological Chemistry·Nancie J SolanCarlos V Paya
Dec 18, 2001·Nature Immunology·Simona SaccaniGioacchino Natoli
Jun 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shoichi SuzukiAtsushi Kumatori
Aug 25, 2004·Science·Federica BenvenutiSebastian Amigorena
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shalin H NaikLi Wu
Aug 19, 2005·Molecular and Cellular Biology·Pedro J CejasKelvin P Lee
Sep 22, 2006·American Journal of Physiology. Cell Physiology·Ying Tian, Michael V Autieri
Jan 12, 2008·Science·Linda M WakimFrancis R Carbone
Nov 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elodie SeguraJosé A Villadangos
Feb 6, 2010·Science·Frederic GeissmannKlaus Ley
Jan 26, 2012·Nature Reviews. Immunology·Gabrielle T Belz, Stephen L Nutt
May 23, 2012·The Journal of Experimental Medicine·Ansuman T SatpathyKenneth M Murphy
Nov 15, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hemant JaiswalPrafullakumar Tailor
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Carlos G BriseñoKenneth M Murphy

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Citations

May 22, 2020·JCI Insight·Diana M ElizondoMichael W Lipscomb
Oct 30, 2020·Biology·Jacopo VizioliPatrizia Pagliara
Mar 7, 2021·International Journal of Molecular Sciences·Mai-Huong Thi NgoYen-Hua Huang

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
PCR
FACS
flow cytometry
transfection
transfect
co-immunoprecipitation
transfections

Software Mentioned

Image Studio
GraphPad Prism
QuantStudio
Flow Jo Jo
ImageJ

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