Allopolyploid origin of Cardamine asarifolia (Brassicaceae): incongruence between plastid and nuclear ribosomal DNA sequences solved by a single-copy nuclear gene

Molecular Phylogenetics and Evolution
Judita LihováKarol Marhold

Abstract

Interspecific hybridization and polyploidization have played central roles in plant diversification. However, technical difficulties in the analyses of low-copy genes have limited the study of the origins of hybrid and polyploid plants. Here, we present a phylogenetic analysis of the hexaploid Cardamine asarifolia, distributed in the southern European Alps and northern Apennines. Our study included all relevant taxa of the genus found in Europe. A marked discrepancy was revealed between the trnL-trnF region of cpDNA and internal transcribed spacer (nrDNA ITS) sequences. To solve the incongruence, we sequenced a single-copy nuclear CHS gene (chalcone synthase) using a novel method to design homoeologue-specific PCR primers to bypass artefacts caused by artificial recombination of homoeologues during PCR and/or cloning. Three homoeologues were isolated from C. asarifolia, providing evidence for its allopolyploid origin. One homoeologue, showing the same phylogenetic position as the ITS sequences, most likely originated from an extinct parent. Furthermore, we documented recurrent polytopic hybridizations between C. asarifolia and diploid C. amara. The allohexaploidization and the following hybridization with a diploid species exem...Continue Reading

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Citations

Aug 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sang-Tae KimMichael J Donoghue
Oct 10, 2008·Molecular Biology and Evolution·Martin A LysakIlia J Leitch
Nov 23, 2006·Molecular Phylogenetics and Evolution·Jeong-Mi ParkGerald M Schneeweiss
Mar 29, 2008·Molecular Phylogenetics and Evolution·Roberta J Mason-Gamer
Mar 19, 2009·Molecular Phylogenetics and Evolution·Ling-Yan JiangGui-Fang Zhao
Sep 1, 2009·American Journal of Botany·Naoko IshikawaHirokazu Tsukaya
Apr 1, 2008·American Journal of Botany·Philippe M FortuneMalika L Ainouche
Mar 13, 2014·Journal of Integrative Plant Biology·Meenakshi Ramaswamy VinithaGeorge Thomas
Jun 11, 2015·PloS One·Reinhold StockenhuberMartin C Fischer
Jun 30, 2016·Molecular Ecology·Rie Shimizu-InatsugiKentaro K Shimizu

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