Allosteric control of hemoglobin S fiber formation by oxygen and its relation to the pathophysiology of sickle cell disease

Proceedings of the National Academy of Sciences of the United States of America
Eric R HenryWilliam A Eaton

Abstract

The pathology of sickle cell disease is caused by polymerization of the abnormal hemoglobin S upon deoxygenation in the tissues to form fibers in red cells, causing them to deform and occlude the circulation. Drugs that allosterically shift the quaternary equilibrium from the polymerizing T quaternary structure to the nonpolymerizing R quaternary structure are now being developed. Here we update our understanding on the allosteric control of fiber formation at equilibrium by showing how the simplest extension of the classic quaternary two-state allosteric model of Monod, Wyman, and Changeux to include tertiary conformational changes provides a better quantitative description. We also show that if fiber formation is at equilibrium in vivo, the vast majority of cells in most tissues would contain fibers, indicating that it is unlikely that the disease would be survivable once the nonpolymerizing fetal hemoglobin has been replaced by adult hemoglobin S at about 1 y after birth. Calculations of sickling times, based on a recently discovered universal relation between the delay time prior to fiber formation and supersaturation, show that in vivo fiber formation is very far from equilibrium. Our analysis indicates that patients survi...Continue Reading

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Citations

Jul 11, 2020·International Journal of Molecular Sciences·Ioannis TsamesidisKarine Reybier
Aug 19, 2020·Blood·Martin H Steinberg
Apr 3, 2021·Experimental Biology and Medicine·Nicola Conran, Stephen H Embury

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Methods Mentioned

BETA
linear dichroism
light-scattering

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