Allosteric control of RNA polymerase by a site that contacts nascent RNA hairpins

Science
I ToulokhonovR Landick

Abstract

DNA, RNA, and regulatory molecules control gene expression through interactions with RNA polymerase (RNAP). We show that a short alpha helix at the tip of the flaplike domain that covers the RNA exit channel of RNAP contacts a nascent RNA stem-loop structure (hairpin) that inhibits transcription, and that this flap-tip helix is required for activity of the regulatory protein NusA. Protein-RNA cross-linking, molecular modeling, and effects of alterations in RNAP and RNA all suggest that a tripartite interaction of RNAP, NusA, and the hairpin inhibits nucleotide addition in the active site, which is located 65 angstroms away. These findings favor an allosteric model for regulation of transcript elongation.

References

Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·K M Meisenheimer, T H Koch
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·D WangR Landick
Aug 14, 1998·Molecular Cell·I SidorenkovM Kashlev
Apr 24, 1999·Science·W S Yarnell, J W Roberts
May 7, 1999·Molecular Cell·I Gusarov, E Nudler
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·T PanT R Sosnick
Sep 28, 1999·Cell·R A Mooney, R Landick
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·I Artsimovitch, R Landick
Aug 1, 2000·Science·N KorzhevaS A Darst
Dec 16, 2000·The EMBO Journal·R D FinnM van Heel

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Citations

Jan 20, 2009·Applied Microbiology and Biotechnology·Vincent RenoufAline Lonvaud-Funel
Feb 15, 2008·Acta Biotheoretica·Jacques DemongeotAndrés Moreira
Nov 11, 2003·Journal of Molecular Biology·Andrew M Ryder, Jeffrey W Roberts
Feb 13, 2002·Current Opinion in Structural Biology·Patrick Cramer
Oct 12, 2010·Biochemistry·Jinwei ZhangAdrian R Ferré-D'Amaré
Feb 17, 2005·Nature Reviews. Molecular Cell Biology·Sandra J Greive, Peter H von Hippel
Apr 14, 2011·Nature Reviews. Molecular Cell Biology·Jason N KuehnerClaire Moore
Apr 12, 2011·Nature Reviews. Microbiology·Thomas J Santangelo, Irina Artsimovitch
Jan 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anastasiya SevostyanovaIrina Artsimovitch
Oct 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alexander V YakhninPaul Babitzke
Apr 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andy H YuanAnn Hochschild
Jun 1, 2010·The Journal of Biological Chemistry·Thomas J Santangelo, John N Reeve
Jul 20, 2010·Nucleic Acids Research·Anastasiya Sevostyanova, Irina Artsimovitch
Aug 13, 2010·Nucleic Acids Research·Lars F WestbladeSeth A Darst
Jan 13, 2012·Nucleic Acids Research·Zhanna BerdygulovaLeonid Minakhin
Oct 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Marina N VassylyevaDmitry G Vassylyev
Sep 15, 2009·Journal of Bacteriology·Thomas J SantangeloJohn N Reeve
Jun 15, 2011·Molecular and Cellular Biology·Murali PalangatRobert Landick
Jul 28, 2011·Molekuliarnaia biologiia·S A Proshkin, A S Mironov
Feb 9, 2007·Doklady. Biochemistry and Biophysics·K D KuznedelovK V Severinov
Apr 16, 2008·Annual Review of Biochemistry·Kristina M HerbertSteven M Block
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Lu BaiMichelle D Wang
Aug 30, 2008·Annual Review of Microbiology·Jeffrey W RobertsJoshua J Filter
May 21, 2003·RNA·Christopher D Cilley, James R Williamson
Mar 7, 2014·Nature Communications·Anssi M MalinenGeorgiy A Belogurov
Jan 10, 2014·Nature·Vitaly EpshteinEvgeny Nudler
Dec 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sergei NechaevE Peter Geiduschek
Mar 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joo-Seop Park, Jeffrey W Roberts
Jan 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yvonne Berghöfer-HochheimerCarol A Gross
Mar 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bryce E NickelsAnn Hochschild
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kaiyu MaWilliam T McAllister
Aug 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alexander V Yakhnin, Paul Babitzke
Aug 12, 2014·Nature Structural & Molecular Biology·Pyae P HeinRobert Landick
Oct 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Padraig DeighanBryce E Nickels
Apr 21, 2012·Chemical Communications : Chem Comm·Satoru NagatoishiNaoki Sugimoto
Jan 30, 2016·Trends in Biochemical Sciences·Jinwei Zhang, Robert Landick
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christophe PennoJohn F Atkins
Aug 31, 2016·Nature Microbiology·Smarajit MondalPaul Babitzke
Mar 29, 2011·Journal of Molecular Biology·Jason M PetersRobert Landick

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