PMID: 11343786May 10, 2001Paper

Allosteric regulation of the cAMP receptor protein

Biochimica Et Biophysica Acta
J G Harman

Abstract

The cyclic AMP receptor protein (CRP) of Escherichia coli is a dimer made up of identical subunits. Each CRP subunit contains a cyclic nucleotide binding pocket and the CRP dimer exhibits negative cooperativity in binding cAMP. In solutions containing cAMP, CRP undergoes sequential conformation changes from the inactive apo-form through the active CRP:(cAMP)(1) complex to the less active CRP:(cAMP)(2) complex depending on the cAMP concentration. Apo-CRP binds DNA with low affinity and no apparent sequence specificity. The CRP:(cAMP)(1) complex exhibits high affinity, sequence-specific DNA binding and interacts with RNA polymerase, whether free in solution or complexed with DNA. The results of genetic, biochemical and biophysical studies have helped to uncover many of the details of cAMP-mediated allosteric control over CRP conformation and activity as a transcription factor. These studies indicate that cAMP binding produces only small, but significant, changes in CRP structure; changes that include subunit realignment and concerted motion of the secondary structure elements within the C-terminal DNA binding domain of each subunit. These adjustments promote CRP surface-patch interaction with RNA polymerase and protrusion of the ...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W EpsteinJ Hesse
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y Kao-HuangP H von Hippel
Mar 1, 1992·Microbiological Reviews·J L Botsford, J G Harman
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D Kahn, D M Crothers
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J KimS Garges
May 20, 1991·Journal of Molecular Biology·S S Zinkel, D M Crothers
Aug 11, 1991·Nucleic Acids Research·Z H YangJ S Krakow
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M Brown, D M Crothers
Mar 5, 1989·Journal of Molecular Biology·D C StraneyD M Crothers
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y L RenJ S Krakow
Dec 26, 1986·Cell·H N Liu-JohnsonD M Crothers
Jan 1, 1985·Annual Review of Biochemistry·W R McClure
Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M EmmerR Perlman
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G ZubayJ Beckwith
Feb 25, 1982·Nucleic Acids Research·P Cossart, B Gicquel-Sanzey
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S H Shanblatt, A Revzin

❮ Previous
Next ❯

Citations

Apr 3, 2012·Applied Microbiology and Biotechnology·Hongfang ZhangRongrong Jiang
Apr 23, 2004·Current Opinion in Structural Biology·Catherine L LawsonRichard H Ebright
Feb 13, 2002·Current Opinion in Structural Biology·Joy L Huffman, Richard G Brennan
Feb 19, 2011·Bioconjugate Chemistry·Daniel ScottSylvia Daunert
Aug 15, 2006·Nature Structural & Molecular Biology·Nataliya PopovychCharalampos G Kalodimos
Nov 19, 2003·European Journal of Biochemistry·Sylwia Kedracka-Krok, Zygmunt Wasylewski
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nataliya PopovychCharalampos G Kalodimos
Jul 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Toshiyuki Ueki, Sumiko Inouye
Feb 6, 2009·The Journal of Biological Chemistry·D Travis GallagherPrasad T Reddy
Sep 11, 2009·The Journal of Biological Chemistry·Manchi C M ReddyJames C Sacchettini
Nov 2, 2005·The Journal of Biological Chemistry·Hwan YounGary P Roberts
Mar 7, 2009·Journal of Experimental Botany·Kaisa MarjamaaKurt V Fagerstedt
Oct 5, 2011·Nucleic Acids Research·Chung-Hang LeungDik-Lung Ma
May 7, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Wenbing TaoShaoning Yu
Feb 25, 2006·Science·Andrew Camilli, Bonnie L Bassler
Jun 15, 2011·Journal of Bacteriology·Timothy J CordesKatrina T Forest
Aug 18, 2009·Journal of Bacteriology·Youjun Feng, John E Cronan
Oct 6, 2009·Journal of Bacteriology·Takayuki Endoh, Joanne N Engel
Sep 20, 2011·Journal of Bacteriology·Sheetal UppalNarendra Jawali
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Pol Nadal JimenezWim J Quax
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Gary P RobertsRobert L Kerby
May 8, 2007·Annual Review of Microbiology·Rita TamayoAndrew Camilli
Sep 22, 2011·PLoS Computational Biology·Simon Mitternacht, Igor N Berezovsky
Sep 22, 2011·PLoS Computational Biology·Jérémie BourdonAnne Siegel
Jul 23, 2013·PLoS Computational Biology·Burcu Aykaç FasTürkan Haliloğlu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.