Allyl Isothiocyanate Ameliorates Dextran Sodium Sulfate-Induced Colitis in Mouse by Enhancing Tight Junction and Mucin Expression

International Journal of Molecular Sciences
Min Woo KimSeung Hyun Oh

Abstract

Inflammatory bowel disease (IBD) is characterized by chronic or recurrent inflammation of the gastrointestinal tract. Even though the current strategies to treat IBD include anti-inflammatory drugs and immune modulators, these treatments have side-effects. New strategies are, therefore, required to overcome the limitations of the therapies. In this study, we investigated the anti-colitic effects of allyl isothiocyanate (AITC), which is an active ingredient present in Wasabia japonica. The DSS-induced colitis model in the mouse was used to mimic human IBD and we observed that AITC treatment ameliorated the severity of colitis. We further studied the mechanism involved to ameliorate the colitis. To investigate the involvement of AITC on the intestinal barrier function, the effect on the intercellular tight junction was evaluated in the Caco-2 cell line while mucin expression was assessed in the LS174T cell line. AITC positively regulated tight junction proteins and mucin 2 (MUC2) against DSS-induced damage or depletion. Our data of in vivo studies were also consistent with the in vitro results. Furthermore, we observed that MUC2 increased by AITC is dependent on ERK signaling. In conclusion, we propose that AITC can be considered...Continue Reading

References

Jan 1, 1983·Critical Reviews in Food Science and Nutrition·G R FenwickW J Mullin
Jan 14, 1999·The American Journal of Physiology·B J van KlinkenJ Dekker
Feb 10, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thomas Y MaHamid M Said
Jan 21, 2006·Alimentary Pharmacology & Therapeutics·L Langmead, D S Rampton
Jul 13, 2006·Gastroenterology·Maria Van der SluisAlexandra W C Einerhand
Apr 10, 2007·The Journal of Surgical Research·Lisa S PoritzWalter A Koltun
Jan 18, 2008·World Journal of Gastroenterology : WJG·Mike G LaukoetterAsma Nusrat
Jul 16, 2008·Inflammatory Bowel Diseases·Michael A McGuckinGraham Radford-Smith
Oct 29, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·Young Sun LeeChun Guang Li
Apr 7, 2009·International Journal of Food Microbiology·Fernando B Luciano, Richard A Holley
Aug 21, 2010·Inflammatory Bowel Diseases·Sa'ad Y Salim, Johan D Söderholm
Jun 23, 2011·Journal of Cellular and Molecular Medicine·Anika Eva WagnerGerald Rimbach
Jun 6, 2012·Journal of Biomedicine & Biotechnology·Martina Perše, Anton Cerar
Feb 1, 2013·Mediators of Inflammation·Yuichi NishiyamaKiyonori Yamaoka
Jun 6, 2013·Gastroenterology Research and Practice·A E DorofeyevR B Kondratiuk
Jan 15, 2014·World Journal of Gastroenterology : WJG·Yi-Zhen Zhang, Yong-Yu Li
Feb 11, 2014·Current Protocols in Immunology·Benoit ChassaingMatam Vijay-Kumar
Jul 31, 2014·Journal of Inflammation Research·Marc FakhouryHani Al-Salami
Jul 4, 2017·International Journal of Molecular Sciences·Lalita SubediSun Yeou Kim

❮ Previous
Next ❯

Citations

Jan 18, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marika CordaroRosanna Di Paola
Aug 23, 2019·World Journal of Gastroenterology : WJG·Hilda Vargas-RoblesMichael Schnoor
Jul 3, 2021·International Journal of Molecular Sciences·Thu Han Le PhanSeung Hyun Oh
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan ZhangNing Li

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis
PCR
confocal microscopy

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here