Allyl isothiocyanate suppresses the proteolytic activation of sterol regulatory element-binding proteins and de novo fatty acid and cholesterol synthesis

Bioscience, Biotechnology, and Biochemistry
Shingo MiyataRyuichiro Sato

Abstract

Sterol regulatory element-binding proteins (SREBPs) are a family of transcription factors that regulate lipid homeostasis by controlling the expression of genes involved in fatty acid and cholesterol synthesis. In this study, we used a stable cell line that expresses a luciferase reporter gene driven by an SRE-containing fatty acid synthase promoter to identify allyl isothiocyanate (AITC), one of the major isothiocyanates in cruciferous vegetables, as a novel SREBP inactivator. We found that AITC downregulated the proteolytic processing of SREBPs and the expression of their target genes in human hepatoma Huh-7 cells. Furthermore, AITC reduced the de novo synthesis of both fatty acids and cholesterol. Our results indicate a novel physiological function of AITC in lipid metabolism regulation.

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Citations

Apr 26, 2019·Bioscience, Biotechnology, and Biochemistry·Masamori IwaseRyuichiro Sato
Apr 9, 2020·Expert Opinion on Biological Therapy·Catherine J Hutchings

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Methods Mentioned

BETA
transgenic
protein assay
PCR

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