Aloin Suppresses Lipopolysaccharide-Induced Inflammatory Response and Apoptosis by Inhibiting the Activation of NF-κB

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Xuan LuoNaihan Xu

Abstract

Numerous herbal-derived natural products are excellent anti-inflammatory agents. Several studies have reported that aloin, the major anthraquinone glycoside obtained from the Aloe species, exhibits anti-inflammatory activity. However, the molecular mechanism of this activity is not well understood. In this report, we found that aloin suppresses lipopolysaccharide-induced pro-inflammatory cytokine secretion and nitric oxide production, and downregulates the expression of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Aloin inhibits the phosphorylation and acetylation of the NF-κB p65 subunit by suppressing the upstream kinases p38 and Msk1, preventing LPS-induced p65 translocation to the nucleus. We have also shown that aloin inhibits LPS-induced caspase-3 activation and apoptotic cell death. Collectively, these findings suggest that aloin effectively suppresses the inflammatory response, primarily through the inhibition of NF-κB signaling.

References

Mar 15, 1993·Biochemical and Biophysical Research Communications·M SarihA Adam
May 20, 1993·The New England Journal of Medicine·J E Parrillo
Aug 1, 1998·The Journal of Biological Chemistry·K B ChoiA Karsan
Jan 12, 2000·Oncogene·C S WilliamsR N DuBois
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D M GerlagG S Firestein
Jan 3, 2001·The Journal of Clinical Investigation·P P Tak, G S Firestein
Nov 7, 2002·The Journal of Biological Chemistry·Rosemary KiernanMonsef Benkirane
Nov 29, 2002·The EMBO Journal·Lin-feng ChenWarner C Greene
Jun 12, 2004·Apoptosis : an International Journal on Programmed Cell Death·T Kuwabara, S Imajoh-Ohmi
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hye Sun ParkYun Soo Bae
Feb 13, 2008·Cell·Matthew S Hayden, Sankar Ghosh
Oct 18, 2008·Nature Reviews. Immunology·Sankar Ghosh, Matthew S Hayden
Apr 9, 2009·Bioscience, Biotechnology, and Biochemistry·Mi-Young ParkMi-Kyung Sung
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Andrea Oeckinghaus, Sankar Ghosh
May 5, 2010·Nitric Oxide : Biology and Chemistry·Andrea PautzHartmut Kleinert
Apr 19, 2011·Nutrition and Cancer·Aaron C TanIqbal Ramzan
Jan 15, 2013·Current Opinion in Immunology·Jueqi Chen, Zhijian J Chen
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Apr 1, 2014·Journal of Photochemistry and Photobiology. B, Biology·Mariane Arnoldi SilvaJuliano Ferreira
Mar 22, 2016·Cells·Frank ChristianRuaidhrí J Carmody

❮ Previous
Next ❯

Citations

Jul 16, 2020·The Journal of Pharmacy and Pharmacology·Da-Bao ChenBiao Cai
Feb 28, 2019·Experimental Physiology·Qing WangCungen Ma
Oct 18, 2020·Tissue Engineering. Part B, Reviews·Jiaheng LiangJingan Li
Jan 19, 2022·Phytotherapy Research : PTR·Jianbin XiaoJingqian Su

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
xenograft
nuclear translocation
PCR
flow cytometry
ubiquitination
enzyme-linked immunosorbent assay
ELISA

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here