Along with its favorable prognostic role, CLCA2 inhibits growth and metastasis of nasopharyngeal carcinoma cells via inhibition of FAK/ERK signaling

Journal of Experimental & Clinical Cancer Research : CR
Yuan-Yuan QiangChao-Nan Qian

Abstract

CLCA2 was reported as a tumor suppressor and disregulated in breast cancer. However, its function in tumor growth and metastasis in NPC has rarely been reported. In this study, we investigated the functional and molecular mechanisms by which CLCA2 influences NPC. CLCA2 expression in human NPC cell lines and tissues was examined via real-time PCR (RT-PCR), Western blot and IHC. The biological roles of CLCA2 in proliferative, migration and invasion of NPC cell lines was evaluated in 5-8F, S18, S26 and SUNE-1 cells. Cell viability, migration and invasion were assessed in vitro by MTS, colony formation and transwell assay, respectively. CLCA2 in growth and metastasis of NPC were evaluated in vivo through NPC xenograft tumor growth, lung metastatic mice model and popliteal lymph node (LN) metastasis model. Overexpression of CLCA2 significantly decreased proliferation, migration and invasion of NPC cells. In contrast, knockdown of CLCA2 elicited the opposite effects. CLCA2 overexpression suppressed xenograft tumor growth and lung, popliteal lymph node (LN) metastasis in vivo. CLCA2 inhibited tumor metastasis through suppressing epithelial-Mesenchymal transition (EMT) and in-activating FAK/ERK1/2 signaling pathway in NPC cells. Immuno...Continue Reading

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Citations

Aug 23, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jun ZouZhi-Gang Jie
Feb 8, 2019·Journal of Experimental & Clinical Cancer Research : CR·Damiano Cosimo RigiraccioloMarcello Maggiolini
Jan 29, 2021·Cytotechnology·Shengpeng ZhangLimin Feng
Jun 5, 2021·Postgraduate Medical Journal·Srikanth Umakanthan, Maryann M Bukelo

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Methods Mentioned

BETA
PCR
Assay
transfections
transfection
xenografts

Software Mentioned

SPSS

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