Aloperine suppresses LPS-induced macrophage activation through inhibiting the TLR4/NF-κB pathway

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Yinyin YeLiangfei Tao

Abstract

The currently available anti-inflammatory drugs often cause diverse side effects with long-term use. Exploring anti-inflammatory drugs with better efficacy and lower toxicity presents an ongoing challenge. Aloperine is an alkaloid extracted from the leaves and seeds of Sophora alopecuroides L. However, the anti-inflammatory effects of Aloperine have not been fully elucidated. This study aimed to investigate whether Aloperine suppresses lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 macrophages. RAW264.7 macrophages were stimulated with LPS (1 μg/mL) in the presence or absence of Aloperine (50 and 100 μM). mRNA expression was measured by real-time PCR, and protein expression was assessed by western blot analysis. The secretion of pro-inflammatory cytokines was measured by ELISA. The levels of nitric oxide (NO) and reactive oxygen species (ROS) were measured by staining. The transcriptional activity of NF-κB was assayed by a luciferase activity assay. The results proved that Aloperine inhibited the expression of LPS-induced pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-17A (IL-17A)] in macrophages. Treatment with Aloperine inhibited NO production through su...Continue Reading

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Citations

Jul 30, 2020·Oxidative Medicine and Cellular Longevity·Ruidong LiKaixong Tao
Mar 20, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Meng-Ting LiZheng-Xin Xu
Jun 4, 2021·Frontiers in Neuroscience·Seung Won Lee, Hee Chul Han
Jun 12, 2021·Journal of Translational Medicine·Dickson Kofi Wiredu OcanseyFei Mao

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Methods Mentioned

BETA
PCR
Assay
ELISA
nuclear translocation

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