PMID: 42917Dec 1, 1979Paper

Alpha- and beta-adrenergic stimulation of arachidonic acid metabolism in cells in culture

Proceedings of the National Academy of Sciences of the United States of America
L Levine, M A Moskowitz

Abstract

Madin-Darby canine kidney cells (MDCK) synthesize prostaglandin (PG) F(2alpha), PGI(2) (measured as 6-keto-PGE(1alpha)), PGE(2), PGD(2), and thromboxane A(2) (measured as thromboxane B(2)). When incubated in the presence of norepinephrine (6 muM), the syntheses of these arachidonic acid metabolites are stimulated 3-fold. Norepinephrine's effect can be antagonized by the addition of alpha-adrenergic receptor blocking agents (phenoxybenzamine>phentolamine>yohimbine>dibenamine>tolazoline) but not by the beta-adrenergic blocking drug propranolol. Norepinephrine's stimulation is also inhibited by low concentrations of dihydroergotamine, bromocryptine, ergocryptine, and ergotamine. The stimulation of PG synthesis by norepinephrine is reversible, continues during the 24 hr of incubation, and requires the presence of norepinephrine at the receptor site but it is not blocked by the addition of colchicine, cytochalasin B, or cycloheximide. Neither phenoxybenzamine nor ergotamine at concentrations that block norepinephrine's stimulation of PG biosynthesis suppresses the increase in PG synthesis induced by exogenous arachidonic acid, suggesting that the alpha-adrenergic regulation is not occurring primarily at the cyclooxygenase step in th...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Hirata, J Axelrod
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F HirataJ Axelrod
Jan 14, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·A JobkeG Hertting
Jan 1, 1968·British Journal of Pharmacology and Chemotherapy·B N DaviesP G Withrington
Nov 1, 1969·British Journal of Pharmacology·J L Laity
Jun 22, 1968·Nature·N GilmoreJ H Wyllie

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Citations

Jan 1, 1987·European Journal of Clinical Pharmacology·A FujimuraA Ebihara
Feb 15, 1983·Biochemical Pharmacology·A A Abdel-LatifR K Dover
Oct 1, 1992·International Journal of Immunopharmacology·A M GenaroE Borda
Jul 1, 1985·Prostaglandins, Leukotrienes, and Medicine·H Peredo, E S Borda
Dec 30, 1981·Biochimica Et Biophysica Acta·J R WilliamsonJ B Hoek
Jan 1, 1982·General Pharmacology·K Hillier, W W Templeton
Mar 24, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jonathan D UrbanRichard B Mailman
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S R CoughlinL Levine
Oct 1, 1980·The Journal of Clinical Investigation·J A OliverP J Cannon
Apr 1, 1986·The Journal of Clinical Investigation·J L NadlerR Horton
Jan 1, 1981·Progress in Lipid Research·L Levine, I Alam
Feb 1, 1982·The American Journal of Medicine·D J LevensonB M Brenner
Feb 15, 1984·Archives of Biochemistry and Biophysics·T YamamotoM Vaughan
May 13, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E S BordaL Sterin-Borda
Feb 1, 1987·Seminars in Arthritis and Rheumatism·D C Brater
May 1, 1990·Clinical Immunology and Immunopathology·G GorelikE S Borda
Mar 1, 1997·Placenta·T YanagawaT Kudo
Sep 1, 1980·Prostaglandins and Medicine·G H RaoJ G White
Jan 1, 1981·Prostaglandins and Medicine·G H RaoJ G White
Aug 1, 1983·American Journal of Obstetrics and Gynecology·M Phillippe
Jul 24, 2001·The Annals of Otology, Rhinology, and Laryngology·B E Sherman, R A Chole
Jan 1, 2015·Autonomic Neuroscience : Basic & Clinical·Kazuo AndoShoshiro Okada
May 1, 1984·Stroke; a Journal of Cerebral Circulation·J A CapraroJ R Little
May 17, 1982·Klinische Wochenschrift·P C WeberR Lorenz

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