Alpha-1-adrenoceptor subtype selective regulation of connexin 43 expression in rat cardiomyocytes

Naunyn-Schmiedeberg's Archives of Pharmacology
Diana Rojas GomezS Dhein

Abstract

Connexin43 (Cx43) is the predominant intercellular gap junction protein in the heart providing intercellular communication for the cell-to-cell transfer of electrical activation. In a previous study, we could show that alpha-adrenoceptor stimulation can affect Cx43 expression and function. We now wanted to elucidate which alpha1-adrenoceptor subtype might be involved. Cultured neonatal rat cardiomyocytes were exposed to various concentrations of phenylephrine (0.1-1,000 nM) for 24 h (n=6). Thereafter, cells were harvested, and after lysis, Cx43 content was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot. Results were normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Finally, we determined the effect of this treatment on intercellular gap junction conductivity using dual whole-cell voltage clamp. Similarly, we tested the effect of an additional treatment with either 10 nM prazosin or, to assess the subtypes, 10 nM of the alpha(1A)-antagonist RS17053 (n=6), 500 nM of the alpha(1B)-antagonist AH1111OA (n=6), or 50 nM of the alpha(1D)-antagonist BMY7378 (n = 6). Moreover, we incubated the cells for 24 h with the alpha(1A)-adrenoceptor agonist A61603 (10 nM). Phenylephrine le...Continue Reading

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Citations

Sep 4, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Yi-Fan YangMing-Jai Su
Feb 15, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Aida Salameh, Stefan Dhein
Jul 19, 2011·Progress in Lipid Research·Hemant PoudyalLindsay Brown
Nov 3, 2009·European Journal of Pharmacology·Roya NaderiMahdieh Faghihi
Nov 4, 2010·Biophysical Journal·Thomas SeidelStefan Dhein
Feb 23, 2011·Journal of Receptor and Signal Transduction Research·Dianne M Perez, Van A Doze
Sep 22, 2017·Pharmacological Reviews·Luc LeybaertRainer Schulz
May 1, 2008·The Journal of Pharmacology and Experimental Therapeutics·Aida SalamehDietrich Pfeiffer

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