PMID: 6164681Apr 1, 1981Paper

alpha 2-macroglobulin adsorbed to colloidal gold: a new probe in the study of receptor-mediated endocytosis

The Journal of Cell Biology
R B DicksonI Pastan

Abstract

alpha 2-Macroglobulin (alpha 2 M) was adsorbed to colloidal gold and used as a new tool in the study of receptor-mediated endocytosis. alpha 2 M-gold is easy to prepare and is clearly visualized at the electron microscope level. When cells were incubated with alpha 2 M-gold at 0 degrees C, gold was visualized both diffusely over the cell surface and concentrated in coated pits. After cells to which alpha 2 M-gold had been bound at 0 degrees C were warmed, the gold was rapidly internalized into uncoated vesicles, previously termed receptosomes. After 30 min of incubation or longer, gold was found in small lysosomes and, later, in large lysosomes and very small vesicles in the region of the Golgi complex. This pattern of localization is similar to that previously described, using peroxidase-labeled anti-alpha 2 M antibodies. By incubating cells with both alpha 2 M-gold and vesicular stomatitis virus (VSV), we studied the internalization of these two markers simultaneously. VSV and alpha 2 M-gold rapidly clustered in the same coated pits and were internalized in the same receptosomes. Proteins and hormones adsorbed to gold may be useful in the study of receptor-mediated endocytosis.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F R MaxfieldI Pastan
Apr 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Horisberger, J Rosset
Feb 1, 1969·Virology·R W SimpsonS Dales
Jul 1, 1972·Vox Sanguinis·M Wickerhauser, Y L Hao
Dec 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·A S Prasad, D Oberleas
Aug 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C WillinghamI Pastan
Jan 1, 1981·Annual Review of Physiology·I H Pastan, M C Willingham

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Citations

Jan 1, 1984·Molecular and Cellular Biochemistry·P J Davies, M P Murtaugh
Jan 1, 1984·Pharmacology & Therapeutics·K C Zoon, H Arnheiter
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M C WillinghamE F Neufeld
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J Y FanL Orci
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R SchlegelI H Pastan
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J FitzGeraldM C Willingham
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R B DicksonI Pastan
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M KriegerD Kingsley
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D TranL Orci
Jul 1, 1985·The Journal of Cell Biology·J W ChenJ T August
Sep 1, 1985·The Journal of Cell Biology·J L CarpentierL Orci
Jul 1, 1982·The Journal of Cell Biology·M C Willingham, I H Pastan
Nov 1, 1983·The Journal of Cell Biology·S Zaremba, J H Keen
Jul 30, 2005·Journal of Virology·Christiane BeerLene Pedersen
Oct 6, 1997·The Journal of Cell Biology·M FotiJ L Carpentier
Dec 1, 1984·Journal of Ultrastructure Research·J S Geoffroy, R P Becker
Apr 1, 1990·Analytical Biochemistry·E Elliott, C Dennison
Jul 29, 1983·Biochemical and Biophysical Research Communications·R Schlegel, M Wade
Oct 15, 1983·Virology·K C ZoonM C Willingham
Dec 1, 1984·The Anatomical Record·J WatanabeM Oka
Jan 1, 1984·Journal of Cellular Biochemistry·D J Yamashiro, F R Maxfield
Oct 15, 1981·Biochemical and Biophysical Research Communications·R SchlegelI Pastan

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