PMID: 8875777Aug 1, 1996Paper

Alpha 2-macroglobulin of rabbits inhibits the habutobin activity

Toxicon : Official Journal of the International Society on Toxinology
U OkaTadayoshi Kosugi

Abstract

We have investigated whether alpha 2-macroglobulin (alpha 2M) of rabbits inhibits the activity of habutobin, a thrombin-like enzyme from Trimeresurus flavoviridis venom. Rabbit alpha 2M was purified with ultracentrifugation, gel filtration on Sepharose 6B and ion exchange chromatography on DEAE-Sephacel. Inhibitory effects of rabbit alpha 2M on habutobin was determined by the fibrin forming activity, digestion of A alpha chain of fibrinogen, and the release of fibrinopeptide A from fibrinogen. As a results, purified alpha 2M showed a single band with high molecular weight, around 800,000 mol. wt by means of polyacrylamide gel electrophoresis using PhastSystem. Besides inhibiting amidolytic and caseinolytic activity of porcine trypsin, it has inhibited the activity of habutobin: that is, in the presence of rabbit alpha 2M, fibrin forming activity of habutobin was decreased and habutobin-induced digestion of A alpha chain was inhibited. In addition, rabbit alpha 2M reduced habutobin-induced release of fibrinopeptide A from rabbit fibrinogen.

References

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Citations

May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·Y TokeshiTadayoshi Kosugi
Sep 7, 2007·Biochemical and Biophysical Research Communications·Masanori SunagawaTadayoshi Kosugi
Mar 5, 1999·Critical Reviews in Toxicology·R HatiK K Bhattacharyya

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