alpha-Adrenergic effects on Na+-K+ pump current in guinea-pig ventricular myocytes

The Journal of Physiology
Y WangG J Baldo

Abstract

1. The whole-cell patch clamp was employed to study Na+-K+ pump current (Ip) in acutely isolated myocytes. alpha-Adrenergic receptors were activated with noradrenaline (NA) after blocking beta-adrenergic receptors with propranolol. Ip was measured as the current blocked by strophanthidin (Str). 2. Activation of alpha-receptors by NA increased Ip in a concentration-dependent manner. The K0.5 depended on intracellular calcium ([Ca2+]i), however maximal stimulation did not. At 15 nM [Ca2+]i the K0.5 was 219 nM NA whereas at 1.4 microM [Ca2+]i it was 3 nM. 3. The voltage dependence of Ip was not shifted by NA at either high or low [Ca2+]i. At each voltage, maximal stimulation of Ip was 14-15 %. 4. Staurosporine (St), an inhibitor of protein kinase C (PKC), eliminated the alpha-receptor-mediated stimulation of Ip at either high or low[Ca2+]i. 5. The stimulation of Ip was independent of changes in intracellular sodium or external potassium concentrations, and did not reflect a change in affinity for Str. 6. Phenylephrine, methoxamine and metaraminol, three selective alpha1-adrenergic agonists, stimulate Ip in a similar manner to NA. Stimulation of Ip by NA was eliminated by prazosin, an alpha1-antagonist, but was unaffected by yohimb...Continue Reading

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Citations

Sep 8, 2012·Cellular and Molecular Life Sciences : CMLS·W FullerK J Wypijewski
Aug 14, 1999·Progress in Biophysics and Molecular Biology·Y Shimoni
Apr 4, 2002·The Journal of General Physiology·Junyuan GaoRichard T Mathias
Mar 19, 2002·The Journal of Physiology·Jean-Daniel Horisberger, Solange Kharoubi-Hess
Mar 17, 2015·The Journal of Physiology·Michael J ShattockZi-Jian Xie
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Aug 4, 2007·Progress in Neurobiology·Tamás L Török
Jan 18, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Jianliang SongJoseph Y Cheung
Nov 30, 2006·Annals of the New York Academy of Sciences·Donald M BersJulie Bossuyt
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Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·K NishimaruK Shigenobu
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·R. T. MathiasY. Wang
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