PMID: 41192Sep 1, 1979

Alpha adrenoceptors in rat brain: direct identification with prazosin

Naunyn-Schmiedeberg's Archives of Pharmacology
R HornungH Glossmann


Tritiated prazosin was used to characterize high affinity binding sites with characteristics similar to alpha 1 adrenoceptors in rat brain membranes. These sites were compared with alpha 2 adrenoceptors labeled with tritiated clonidine. The prazosin sites had an association constant of 2 nM-1 and bound to ligand optimal around pH 7.0. The density of the sites was 300 fmoles per mg of protein; the half time of dissociation of prazosin was 7 min at 30 degrees C. The order or potencies of agonists, determined from binding-inhibition experiments with labeled prazosin, was: naphazoline greater than clonidine greater than adrenaline greater than noradrenaline greater than phenylephrine greater than alpha-methylnoradrenaline greater than dopamine. The order of potencies of antagonists was: prazosin greater than phenoxybenzamine greater than phentolamine greater than clozapine greater than yohimbine. Sodium ions and divalent cations as well as guanyl nucleotides have little or no effect on the binding of the labeled antagonist. This is in contrast to the binding of the labeled agonist clonidine (Glossmann and Presek, 1979a, 1979b). Labeled prazosin may be a useful tool to characterize alpha 1 adrenoceptors.


Jun 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·G Gross, I Lues
Dec 1, 1982·Naunyn-Schmiedeberg's Archives of Pharmacology·B RouotJ Schwartz
Dec 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·H GlossmannP Bellemann
Nov 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·H W WetzelS Z Langer
Nov 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·D B MenkesG K Aghajanian
Nov 1, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·T Tanaka, K Starke
Feb 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·M J MorrisP Meyer
Jun 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·H Glossman, R Hornung
Jun 1, 1994·Naunyn-Schmiedeberg's Archives of Pharmacology·S M YuC M Teng
Dec 1, 1986·Electroencephalography and Clinical Neurophysiology·R S Neuman
Jul 17, 1981·European Journal of Pharmacology·G Engel, D Hoyer
Dec 17, 1982·European Journal of Pharmacology·M R Dashwood
Jan 28, 1983·European Journal of Pharmacology·D B MenkesD W Gallager
Nov 27, 1984·European Journal of Pharmacology·K YamanakaI Muramatsu
Aug 17, 1984·European Journal of Pharmacology·E Richelson, A Nelson
Jan 2, 1985·European Journal of Pharmacology·F HataH Ishida
Feb 26, 1985·European Journal of Pharmacology·A L MorrowI Creese
Jan 29, 1986·European Journal of Pharmacology·K E DickinsonP S Sever
Dec 1, 1982·Pharmacology, Biochemistry, and Behavior·T V DunwiddieT Worth
Apr 1, 1982·Pharmacology, Biochemistry, and Behavior·B A Pappas, R Ings
May 1, 1985·Pharmacology, Biochemistry, and Behavior·J R UnnerstallL M Orensanz
Dec 1, 1988·Pharmacology, Biochemistry, and Behavior·G E LeightonJ Hughes
Mar 1, 1989·Pharmacology, Biochemistry, and Behavior·D W Herr, R B Mailman
Feb 1, 1981·Journal of the Autonomic Nervous System·R B McCall, S J Humphrey
Jan 1, 1986·Neurochemistry International·A L MorrowI Creese
Jul 1, 1995·Neurobiology of Aging·M Eriksdotter JönhagenJ Luthman
Sep 1, 1980·Molecular and Cellular Endocrinology·H Glossmann, R Hornung
Nov 12, 1990·Molecular and Cellular Endocrinology·R E McGeheeL E Cornett
Oct 10, 1983·Neuroscience Letters·T C Rainbow, A Biegon


Dec 17, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·W KrawietzC Konrad
Jan 1, 1977·Reviews of Physiology, Biochemistry and Pharmacology·K Starke
May 1, 1977·British Journal of Pharmacology·J C DoxeyJ M Walker
Sep 1, 1977·Life Sciences·S Berthelsen, W A Pettinger
Dec 9, 1977·Brain Research·D C U'PrichardS H Snyder
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Titeler, P Seeman
Mar 31, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·K StarkeE Borowski
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A J Blume
Dec 27, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·J KleinsteinC Konrad
Jan 31, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·H Glossmann, P Presek
Feb 1, 1979·The New England Journal of Medicine·J Koch-WeserW A Pettinger

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