Alpha-catenin-dependent recruitment of the centrosomal protein CAP350 to adherens junctions allows epithelial cells to acquire a columnar shape

PLoS Biology
Maria P GavilanRosa M Rios

Abstract

Epithelial morphogenesis involves a dramatic reorganisation of the microtubule cytoskeleton. How this complex process is controlled at the molecular level is still largely unknown. Here, we report that the centrosomal microtubule (MT)-binding protein CAP350 localises at adherens junctions in epithelial cells. By two-hybrid screening, we identified a direct interaction of CAP350 with the adhesion protein α-catenin that was further confirmed by co-immunoprecipitation experiments. Block of epithelial cadherin (E-cadherin)-mediated cell-cell adhesion or α-catenin depletion prevented CAP350 localisation at cell-cell junctions. Knocking down junction-located CAP350 inhibited the establishment of an apico-basal array of microtubules and impaired the acquisition of columnar shape in Madin-Darby canine kidney II (MDCKII) cells grown as polarised epithelia. Furthermore, MDCKII cystogenesis was also defective in junctional CAP350-depleted cells. CAP350-depleted MDCKII cysts were smaller and contained either multiple lumens or no lumen. Membrane polarity was not affected, but cortical microtubule bundles did not properly form. Our results indicate that CAP350 may act as an adaptor between adherens junctions and microtubules, thus regulatin...Continue Reading

References

Dec 1, 1990·The Journal of Cell Biology·M H BréE Karsenti
Nov 1, 2000·Nature Cell Biology·A ChausovskyG G Borisy
Oct 5, 2001·Nature Cell Biology·L A LigonE L Holzbaur
Feb 2, 2002·Nature Reviews. Genetics·Joachim WittbrodtManfred Schartl
Nov 6, 2002·The Journal of Biological Chemistry·Helene MarieVania M M Braga
Jun 24, 2004·Mechanisms of Development·Takashi Iwamatsu
Nov 30, 2005·Molecular Biology of the Cell·Xiumin YanErich A Nigg
Apr 13, 2006·Journal of Cell Science·Samantha J StehbensAlpha S Yap
Jan 18, 2007·The Journal of Cell Biology·Terry Lechler, Elaine Fuchs
Sep 20, 2007·Journal of Cell Science·Aurélie Hoppeler-LebelAnne-Marie Tassin
Aug 5, 2008·Cell Cycle·Michael ShtutmanAlexander D Bershadsky
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Wenxiang Meng, Masatoshi Takeichi
May 19, 2010·The Journal of Cell Biology·Alejo E Rodriguez-FraticelliFernando Martín-Belmonte
Jun 22, 2010·Molecular & Cellular Proteomics : MCP·W Y TongY W Lam
Aug 17, 2011·The Journal of Cell Biology·Kaelyn D SumigrayTerry Lechler
Nov 16, 2011·The Journal of Cell Biology·Stephanie L Maiden, Jeff Hardin
Dec 14, 2011·Journal of Cell Science·Juan HuangYang Hong
Apr 5, 2012·Molecular and Cellular Biology·Veenu TripathiDrazen B Zimonjic
Jun 19, 2012·Current Opinion in Cell Biology·Kenji Sugioka, Hitoshi Sawa
Jul 4, 2012·Nature Cell Biology·Aparna RatheeshAlpha S Yap
Jul 4, 2012·Bioarchitecture·Kaelyn D Sumigray, Terry Lechler
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nobutoshi TanakaMasatoshi Takeichi
Dec 1, 2012·Nature Cell Biology·Gerard ApodacaDavid M Bryant
Dec 25, 2012·Nature Cell Biology·Nasreen Akhtar, Charles H Streuli
Jan 8, 2013·Nature Structural & Molecular Biology·Erumbi S Rangarajan, Tina Izard
Jan 22, 2013·Seminars in Cell & Developmental Biology·Magdalene Michael, Alpha S Yap
Dec 26, 2013·The Journal of Cell Biology·Marta N ShahbaziMirna Perez-Moreno
Aug 20, 2014·Nature Communications·Thibaut EguetherAnne-Marie Tassin

❮ Previous
Next ❯

Citations

Nov 1, 2016·Cell Structure and Function·Mika Toya, Masatoshi Takeichi
Feb 22, 2017·Biology of the Cell·Sophie SluysmansSandra Citi
Dec 30, 2017·The Journal of Cell Biology·Jadranka Loncarek, Mónica Bettencourt-Dias
Nov 6, 2018·Tissue Barriers·Ekaterina Vasileva, Sandra Citi
Oct 18, 2019·Biology of the Cell·Michel Bornens
Mar 31, 2017·F1000Research·Vivian Tang
Oct 17, 2018·Nature Reviews. Molecular Cell Biology·Marileen Dogterom, Gijsje H Koenderink

❮ Previous
Next ❯

Datasets Mentioned

BETA
FM166216

Methods Mentioned

BETA
two-hybrid
co-immunoprecipitation
co-IP
transfection
fluorescence-activated cell-sorting
confocal microscopy
PCR
flow cytometry
FCS
FACS

Software Mentioned

Leica
ImageJ
Adobe Photoshop
Gene Tools
Elements
BLAST
Metamorph Offline
NIS
GraphPad Prism
Image Quant

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.