Alpha-crystallin acting as a molecular chaperone protects catalase against steroid-induced inactivation

FEBS Letters
D W Hook, J J Harding

Abstract

A link between corticosteroid therapy and the development of cataract has been known for many years. However, the precise underlying molecular mechanism of pathology has not been characterised, although a role for direct deleterious interactions between corticosteroids and lenticular proteins has been investigated. Alpha-crystallin is a major lens protein that has exhibited chaperone properties in vitro. Catalase is a ubiquitous enzyme that is an important scavenger of hydrogen peroxide in vivo. The corticosteroid prednisolone-21-hemisuccinate was found to inactivate bovine liver catalase, in vitro in a progressive manner. Coincubation of alpha-crystallin with catalase in a 1:2 molar ratio (one alpha-crystallin to two catalase molecules) fully protected against this inactivation. The protection was specific. Aspirin-like analgesics, putative anti-cataract drugs offered no such protection.

References

Apr 1, 1992·Experimental Eye Research·R Blakytny, J J Harding
Feb 11, 1991·Archives of Ophthalmology·M C LeskeS Y Wu
Oct 1, 1989·Acta Ophthalmologica·J J HardingR S Harding
Jan 16, 1989·Biochemical and Biophysical Research Communications·S P Bhat, C N Nagineni
Sep 28, 1987·FEBS Letters·R C Augusteyn, J F Koretz
Jun 1, 1985·Experimental Eye Research·R BucalaA Cerami
Oct 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·M E Percy
Feb 7, 1981·Lancet·E Cotlier, Y R Sharma
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T D Ingolia, E A Craig
Nov 1, 1984·The Journal of Clinical Investigation·S ManabeA Cerami
Oct 25, 1981·Journal of Molecular Biology·M R MurthyM G Rossmann
Aug 1, 1994·Experimental Eye Research·J A CarverR J Truscott
Nov 11, 1993·International Journal of Cancer. Journal International Du Cancer·A AoyamaR Klemenz
Sep 10, 1960·JAMA : the Journal of the American Medical Association·R L BLACKJ J BUNIM

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Citations

Apr 1, 2000·Journal of the American Aging Association·L S Luckinbill, P Foley
Jul 3, 1998·International Journal of Biological Macromolecules·D W Hook, J J Harding
Apr 27, 1999·Progress in Retinal and Eye Research·B K Derham, J J Harding
Jun 18, 2002·Ageing Research Reviews·John J Harding
Jan 22, 2000·The Biochemical Journal·E Ganea, J J Harding
Jul 1, 1997·European Journal of Biochemistry·D W Hook, J J Harding
May 24, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·André-Patrick Arrigo, Benjamin Gibert
Jun 16, 2005·Biochimica Et Biophysica Acta·Hong Yan, John J Harding
Nov 8, 1996·The Journal of Biological Chemistry·J Durner, D F Klessig
Jul 18, 1997·Biochemical and Biophysical Research Communications·B K Das, J J Liang
Jul 30, 1999·Biochemical and Biophysical Research Communications·F A BettelheimJ S Zigler
Jun 25, 1999·Biochimica Et Biophysica Acta·H Yan, J J Harding
Jul 3, 2021·Nanomaterials·Belal I HanafyBarbara K Pierscionek
Sep 12, 2006·Biochimica Et Biophysica Acta·John J Harding, Elena Ganea

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