PMID: 9188067May 1, 1997Paper

alpha-Crystallin acting as a molecular chaperonin against photodamage by UV irradiation

Journal of Protein Chemistry
J S LeeS H Chiou

Abstract

alpha-Crystallin, a major protein of the eye lens, is known to have chaperone activity in preventing heat-induced aggregation of enzymes and other crystallins. In this study, we investigate the ability of alpha-crystallin to inhibit UV-light-induced aggregation of other lens proteins and the effect of exposure of alpha-crystallin to UV irradiation on its chaperone activity. The chaperone activities of alpha-crystallin preincubated at different temperatures were found to be different and could be correlated with its change in quaternary structure as determined by the fluorescence probe ANS (8-anilo-1-naphthalene sulfonate). alpha-Crystallin can inhibit the aggregation of gamma-crystallin from UV irradiation at room temperature, and the preheated alpha-crystallins provide more protection than the native one. Upon irradiation by UV light, alpha-crystallin gradually lost its ability to protect beta-crystallin against thermal aggregation. The loss of the chaperone efficacy of alpha-crystallin to protect other lens proteins may shed light on human cataract formation induced by long-term exposure to UV irradiation.

Citations

May 16, 1998·The Journal of Biological Chemistry·K K SharmaK Kester
May 26, 2017·PloS One·Sibel CetinelCarlo Montemagno
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G PelusoM Calvani
Nov 27, 2018·Biochemistry. Biokhimii︠a︡·L K MuranovaN B Gusev
Apr 25, 2009·IUBMB Life·Pasupulati Anil Kumar, Geereddy Bhanuprakash Reddy
Feb 29, 2000·The Journal of Biological Chemistry·B A Cobb, J M Petrash
Feb 22, 2013·Photochemistry and Photobiology·Viktor PajerAntal Nógrádi
Mar 26, 1998·Biochemical and Biophysical Research Communications·J S LeeS H Chiou
Mar 7, 2002·Experimental Eye Research·Miriam Hernández-QuinteroFederico Castro-Muñozledo
Jul 20, 2002·Biochemical and Biophysical Research Communications·Jiahn Haur LiaoShyh Horng Chiou
Feb 27, 2003·Chemico-biological Interactions·Rizwan ManzerVasilis Vasiliou
Jun 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shu-Shun HsuehSteven S-S Wang

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