Alpha-galactosyl epitope-mediated activation of porcine aortic endothelial cells: type I activation

Transplantation
A PalmetshoferF H Bach

Abstract

The galactose alpha(1-3)galactose (alpha-gal) epitope associated with membrane glycoproteins and glycolipids represents a major determinant recognized on porcine cells by human xenoreactive natural antibodies (XNA). Together, bound XNA and complement rapidly induce porcine aortic endothelial cell (PAEC) activation; this process is associated with cellular shape changes, transient development of intercellular gaps, and loss of ATDPase and thrombomodulin, with release of heparan sulfate. The aim of this study was to evaluate patterns of type I endothelial cell activation (i.e., activation that does not require protein synthesis) following ligation of alpha-gal epitopes with anti-Gal antibodies and alpha-gal-specific lectins. PAEC incubated in the presence of the alpha-gal binding, Bandeiraea simplicifolia lectin (BS-I) underwent cellular shape changes associated with the formation of intercellular gaps. PAEC exposure to BS-I was also associated with the tyrosine phosphorylation of a protein (apparent molecular mass of approximately 130 kDa), not observed following lipopolysaccharide, tumor necrosis factor, or XNA stimulation. This lectin-induced tyrosine phosphorylation was not affected by cytochalasin D (inhibitor of actin filam...Continue Reading

References

Apr 1, 1990·Physiological Reviews·J S Pober, R S Cotran
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J KornbergR L Juliano
Feb 1, 1991·The Journal of Cell Biology·M G LampugnaniP C Marchisio
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·U GaliliB A Macher
Nov 1, 1984·The Journal of Experimental Medicine·U GaliliI Flechner
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M S SandrinI F McKenzie
Feb 1, 1994·The Journal of Experimental Medicine·M A ReadT Collins
Sep 1, 1994·European Journal of Immunology·K M StuhlmeierF H Bach
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Richardson, J T Parsons
Jan 1, 1995·The Journal of Experimental Medicine·S Saadi, J L Platt
Jan 6, 1995·The Journal of Biological Chemistry·N MorinoY Nojima
Oct 1, 1994·Immunological Reviews·J L Platt
Oct 1, 1994·Immunological Reviews·D K CooperR Oriol
Oct 1, 1994·Immunological Reviews·F H BachW W Hancock
Jul 1, 1995·Immunobiology·M L Schmitz, P A Baeuerle
Feb 1, 1996·Current Opinion in Structural Biology·M Chytil, G L Verdine
Oct 4, 1996·Cell·P A Baeuerle, D Baltimore
Mar 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·M Introna, A Mantovani

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Citations

Aug 16, 2002·Transplant Immunology·Anja Roos, Mohamed R Daha
Sep 19, 2003·Chest Surgery Clinics of North America·Thomas K Waddell, Mark D Peterson
Apr 3, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Martin J GoddardJohn Wallwork
Dec 15, 2005·Heart, Lung & Circulation·Karen M DwyerAnthony J F d'Apice
Apr 13, 1999·Transplantation·I F McKenzieM S Sandrin
Jan 2, 2007·Transplantation·Guerard W ByrneChristopher G A McGregor
Dec 16, 1998·Xenotransplantation·M Koulmanda, H Auchincloss
Jul 23, 1999·Annals of the New York Academy of Sciences·S C RobsonF H Bach
Sep 24, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Anthony Dorling
Jun 16, 2004·Xenotransplantation·Abhilash P ChandraPhilip J O'Connell
Jun 16, 2004·Xenotransplantation·Bernd GollacknerSimon C Robson
Mar 12, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·John F GrehanAgustin P Dalmasso
Apr 9, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Guerard W ByrneChristopher G A McGregor
Jul 20, 2006·Clinical Transplantation·Akira Shimizu, Kazuhiko Yamada
Aug 15, 1998·Biochemical and Biophysical Research Communications·M von AlbertiniS C Robson
Jul 5, 2005·Expert Opinion on Investigational Drugs·L E Diamond
May 5, 2001·Immunity·M Cascalho, J L Platt
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A P DalmassoM S Abrahamsen
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hui XuAnita S-F Chong
Apr 11, 2020·Advanced Drug Delivery Reviews·Dong NiuGeorge M Church

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