PMID: 9164838May 15, 1997Paper

alpha-Glucosidase and N-acetylglucosamine-6-sulphatase are the major mannose-6-phosphate glycoproteins in human urine

The Biochemical Journal
David E SleatP Lobel

Abstract

Most newly synthesized lysosomal enzymes contain a transient carbohydrate modification, mannose 6-phosphate (Man-6-P), which signals their vesicular transport from the Golgi to the lysosome via Man-6-P receptors (MPRs). We have examined Man-6-P glycoproteins in human urine by using a purified soluble fragment of the soluble cation-independent MPR (sCI-MPR) as a preparative and analytical affinity reagent. In a survey of urine samples from seven healthy subjects, the pattern of Man-6-P glycoproteins detected with iodinated sCI-MPR as a probe in a blotting assay was essentially identical in each, regardless of sex or age. Two bands of approx. 100 and 110 kDa were particularly prominent. Man-6-P glycoproteins in human urine were purified by affinity chromatography on immobilized sCI-MPR. Seven distinct bands revealed by SDS/PAGE and Coomassie Blue staining were subjected to N-terminal sequence analysis. The prominent 100 and 110 kDa Man-6-P glycoproteins were identified as N-acetylglucosamine-6-sulphatase and alpha-glucosidase respectively. This identification was confirmed by molecular mass determinations on the two major bands after deglycosylation. Sequence analysis revealed arylsulphatase A and several previously unidentified ...Continue Reading

Citations

Sep 1, 2007·Proteomics. Clinical Applications·David E SleatPeter Lobel
Apr 20, 2018·The Journal of Biological Chemistry·Alexander ZwirzitzVladimir Leksa
Jan 10, 2006·Molecular & Cellular Proteomics : MCP·David E SleatPeter Lobel
Nov 18, 2000·Electrophoresis·A JournetJ Garin
Oct 30, 2020·The Biochemical Journal·Torben Lübke, Markus Damme
May 30, 2008·Journal of Proteome Research·David E SleatPeter Lobel
Aug 14, 1999·Journal of the American Society of Nephrology : JASN·M Goto, K Mizunashi
Jan 30, 2003·European Journal of Cell Biology·Virginie SantilmanFrancis Mainferme
Sep 4, 2021·Molecular Omics·Pathma Muthukottiappan, Dominic Winter
Nov 4, 2008·Biochimica Et Biophysica Acta·Torben LübkeDavid E Sleat

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