Alpha-linolenic acid protects the developmental capacity of bovine cumulus-oocyte complexes matured under lipotoxic conditions in vitro

Biology of Reproduction
Waleed F MareiJ L M R Leroy

Abstract

Elevated concentrations of free fatty acids (FFAs), predominantly palmitic, stearic, and oleic acids (PSO), exert detrimental effects on oocyte developmental competence. This study examined the effects of omega-3 alpha-linolenic acid (ALA) during in vitro oocyte maturation (IVM) in the presence of PSO on subsequent embryo development and quality, and the cellular mechanisms that might be involved. Bovine cumulus-oocyte complexes (COCs) were supplemented during IVM with ALA (50 μM), PSO (425 μM), or PSO+ALA. Compared with FFA-free controls (P < 0.05), PSO increased embryo fragmentation and decreased good quality embryos on day 2 postfertilization. Day 7 blastocyst rate was also reduced. Day 8 blastocysts had lower cell counts and higher apoptosis but normal metabolic profile. In the PSO group, cumulus cell (CC) expansion was inhibited with an increased CC apoptosis while COC metabolism was not affected. Mitochondrial inner membrane potential (MMP; JC-1 staining) was reduced in the CCs and oocytes. Heat shock protein 70 (HSP70) but not glucose-regulated protein 78 kDa (GRP78, known as BiP; an endoplasmic reticulum stress marker) was upregulated in the CCs. Higher reactive oxygen species levels (DCHFDA staining) were detected in t...Continue Reading

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Citations

Apr 19, 2018·Biology of Reproduction·Katrin GillerSusanne E Ulbrich
Jan 1, 2019·Reproduction, Fertility, and Development·Bernard A J Roelen
Apr 23, 2020·Cells·Rozenn Dalbies-TranSvetlana Uzbekova
Jun 9, 2020·Reproductive Biology and Endocrinology : RB&E·Vijay Simha BaddelaJens Vanselow
Nov 29, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Débora R GonçalvesWaleed F A Marei
Nov 6, 2020·International Journal of Molecular Sciences·Anouk SmitsWaleed F A Marei
Apr 4, 2021·International Journal of Molecular Sciences·Fabiana de Andrade Melo-Sterza, Ralf Poehland
Sep 16, 2020·Ecotoxicology and Environmental Safety·Feifei ShiXizeng Feng

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