Alpha lipoic acid reverses the negative effect of LPS on mouse spermatozoa and developmental competence of resultant embryos in vitro

Andrology
A MakvandiM H Nasr-Esfahani

Abstract

Reproductive toxicity of lipopolysaccharide (LPS) on spermatozoa is well established. The aim of the present study was to show the potential benefits of alpha lipoic acid (ALA) as a strong antioxidant in alleviating the reproductive toxicity of LPS. Sperm cells and cumulus-oocyte complexes (COCs) were collected from healthy NMRI mice (body weights ranged from 25 to 35 g, 100 females and 200 males). Sperm cells were treated with varying doses of ALA (0.01, 0.02, and 0.04 mm) and 0.01 μg/mL of LPS for 4 h. The quality of spermatozoa (ROS production, DNA fragmentation, and spontaneous acrosome reaction), sperm fertilizability, and the consequent developmental competence of oocytes inseminated with ALA/LPS-treated spermatozoa were recorded. The results showed that 0.04 mm of ALA abrogated LPS-reduced sperm motility, viability, ROS production, spontaneous acrosome reaction, fertilizability, and developmental competence. In addition, 0.04 mm ALA significantly reverted the negative effects of LPS on inner cell mass (ICM) cell counts, total cell number (TCM), and ratio between ICM and TCM. Our data showed that ALA significantly could abrogate the negative effects of LPS on sperm quality and oocyte developmental competence. Therefore, A...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C G FragaB N Ames
Jan 1, 1995·Reproduction, Fertility, and Development·R J Aitken
Dec 1, 1996·Urology·R K Sharma, A Agarwal
Apr 1, 1997·International Journal of Andrology·J F Griveau, D Le Lannou
May 26, 1999·Journal of Reproduction and Fertility·R J Aitken
May 29, 2001·Current Medicinal Chemistry·S C Sikka
Jul 5, 2001·Fertility and Sterility·K UrataI Miyakawa
Aug 10, 2002·Journal of Assisted Reproduction and Genetics·Irma Virant-KlunHelena Meden-Vrtovec
Aug 23, 2008·Clinics·Siti Fatimah IbrahimMohd Padzil Abdul Rahman
Jan 5, 2011·Chemico-biological Interactions·Abdelkader E AshourAdel R A Abd-Allah
Mar 5, 2013·Current Biology : CB·Kiyoshi Miki, David E Clapham
Oct 24, 2013·Current Opinion in Microbiology·Ge ZhangDaniel Kahne
Mar 10, 2015·Systems Biology in Reproductive Medicine·Mahsa BahreinianMohammad H Nasr-Esfahani
Apr 3, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Noritaka HirohashiMariano G Buffone
Apr 10, 2015·Andrology·Jin KimMyung-Geol Pang
Jan 20, 2016·The Tohoku Journal of Experimental Medicine·Zhongyuan LiXiaoping Zhang

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Citations

Oct 11, 2019·Dose-response : a Publication of International Hormesis Society·Lei Wang, Lili Zhuang
Jan 11, 2021·Reproduction in Domestic Animals = Zuchthygiene·Yuanyuan HeFusheng Quan

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