Alpha lipoic acid selectively inhibits proliferation and adhesion to fibronectin of v-H-ras-transformed 3Y1 cells.

Journal of Clinical Biochemistry and Nutrition
Masao YamasakiKazuo Nishiyama

Abstract

Here, we focused on the effects of racemic α-lipoic acid on proliferation and adhesion properties of 3Y1 rat fibroblasts and the v-H-ras-transformed derivative, HR-3Y1-2 cells. Racemic α-lipoic acid inhibited proliferation of HR-3Y1-2 but not 3Y1 cells at 0.3 and 1.0 mM. R-(+)-α-lipoic acid also inhibited proliferation of HR-3Y1-2 cells equivalent to that of racemic α-lipoic acid. In addition, racemic α-lipoic acid decreased intracellular reactive oxygen species levels in HR-3Y1 cells but not 3Y1 cells. Next, we evaluated the effects of racemic α-lipoic acid on cell adhesion to fibronectin. The results indicated that racemic α-lipoic acid decreased adhesive ability of HR-3Y1-2 cells to fibronectin-coated plates. As blocking antibody experiment revealed that β1-integrin plays a key role in cell adhesion in this experimental system, the effects of racemic α-lipoic acid on the expression of β1-integrin were examined. The results indicated that racemic α-lipoic acid selectively downregulated the expression of cell surface β1-integrin expression in HR-3Y1-2 cells. Intriguingly, exogenous hydrogen peroxide upregulated cell surface β1-integrin expression in 3Y1 cells. Taken together, these data suggest that reduction of intracellular ...Continue Reading

References

Aug 1, 1995·Free Radical Biology & Medicine·L PackerH J Tritschler
Mar 14, 1997·Science·K IraniP J Goldschmidt-Clermont
Jun 2, 1998·Free Radical Biology & Medicine·J BustamanteB H Rihn
Feb 14, 2002·Archives of Biochemistry and Biophysics·Hadi MoiniLester Packer
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jung H SuhTory M Hagen
Jun 10, 2004·British Journal of Cancer·S BamfordR Wooster
Apr 22, 2005·Apoptosis : an International Journal on Programmed Cell Death·U WenzelH Daniel
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·S A MarshJ S Coombes
Feb 1, 2006·Traffic·Patrick T Caswell, Jim C Norman
Mar 8, 2006·Journal of Herbal Pharmacotherapy·Jerry Robert SmithKeith K Parker
Dec 1, 2006·Apoptosis : an International Journal on Programmed Cell Death·G SimbulaM Pibiri
Mar 27, 2007·Annals of the New York Academy of Sciences·Soon Ok ChoHyeyoung Kim
Mar 27, 2007·Nature Reviews. Cancer·Suzanne SchubbertGideon Bollag
Nov 21, 2007·Biochimica Et Biophysica Acta·Amine SadokHervé Kovacic
Dec 27, 2007·Antioxidants & Redox Signaling·Heidi C O'NeillCarl W White
Jun 24, 2008·Nature Reviews. Molecular Cell Biology·Antoine E Karnoub, Robert A Weinberg
Jan 2, 2009·In Vitro Cellular & Developmental Biology. Animal·Masao YamasakiKazuo Nishiyama
Dec 24, 2009·Nature Reviews. Cancer·Jay S Desgrosellier, David A Cheresh

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Citations

Jan 16, 2014·Journal of Clinical Biochemistry and Nutrition·Masao YamasakiKazuo Nishiyama
Oct 7, 2014·Expert Review of Clinical Pharmacology·Paul M BinghamZuzana Zachar
Mar 31, 2015·Biochemical and Biophysical Research Communications·Ekaterina BozhokinaIrina Gamaley

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Methods Mentioned

BETA
protein assay
electrophoresis

Software Mentioned

Statcel2
MultiCycle

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