Alpha-lipoic acid supplementation protects enzymes from damage by nitrosative and oxidative stress

Biochimica Et Biophysica Acta
Sylvia HillerXianwen Yi

Abstract

S-nitrosylation of mitochondrial enzymes involved in energy transfer under nitrosative stress may result in ATP deficiency. We investigated whether α-lipoic acid, a powerful antioxidant, could alleviate nitrosative stress by regulating S-nitrosylation, which could result in retaining the mitochondrial enzyme activity. In this study, we have identified the S-nitrosylated forms of subunit 1 of dihydrolipoyllysine succinyltransferase (complex III), and subunit 2 of the α-ketoglutarate dehydrogenase complex by implementing a fluorescence-based differential quantitative proteomics method. We found that the activities of these two mitochondrial enzymes were partially but reversibly inhibited by S-nitrosylation in cultured endothelial cells, and that their activities were partially restored by supplementation of α-lipoic acid. We show that protein S-nitrosylation affects the activity of mitochondrial enzymes that are central to energy supply, and that α-lipoic acid protects mitochondrial enzymes by altering S-nitrosylation levels. Inhibiting protein S-nitrosylation with α-lipoic acid seems to be a protective mechanism against nitrosative stress. Identification and characterization of these new protein targets should contribute to expa...Continue Reading

References

Jan 1, 1992·British Journal of Pharmacology·R M PalmerS Moncada
Dec 15, 1981·The Biochemical Journal·G J CooneyE A Newsholme
Aug 1, 1995·Free Radical Biology & Medicine·L PackerH J Tritschler
Aug 2, 1996·The Journal of Biological Chemistry·R J SinghB Kalyanaraman
Dec 6, 1996·The Journal of Biological Chemistry·T MacalmaM C Beckerle
Mar 26, 1998·Protein Science : a Publication of the Protein Society·L J Reed
Apr 3, 2001·The Journal of Biological Chemistry·A C Nulton-Persson, L I Szweda
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·S R Jaffrey, S H Snyder
Jul 2, 2002·The Journal of Biological Chemistry·Harvey E Marshall, Jonathan S Stamler
Feb 8, 2003·Science·Enzo NisoliMichele O Carruba
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Isabella Dalle-DonneRoberto Colombo
Sep 19, 2003·Current Infectious Disease Reports·David Brealey, Mervyn Singer
Sep 17, 2004·Endothelium : Journal of Endothelial Cell Research·Fan JiangGregory J Dusting
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Douglas T HessJonathan S Stamler
Sep 2, 2005·Molecular and Cellular Biology·Xianwen Yi, Nobuyo Maeda
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laszlo Tretter, Vera Adam-Vizi
Mar 8, 2006·Journal of Herbal Pharmacotherapy·Jerry Robert SmithKeith K Parker
Mar 3, 2007·Circulation Research·Nageswara R Madamanchi, Marschall S Runge
Jan 5, 2008·Infection and Immunity·Nicole M IovineYvette Weinrauch
Oct 10, 2008·Journal of Bioenergetics and Biomembranes·Maik HüttemannJeffrey W Doan
Oct 10, 2009·Nature Protocols·Mariusz R WieckowskiPaolo Pinton
Jan 23, 2010·Free Radical Biology & Medicine·Brian P DrankaVictor M Darley-Usmar
Feb 6, 2010·Circulation Research·Junhui Sun, Elizabeth Murphy
Mar 6, 2010·Circulation Research·Brian LimaJonathan S Stamler
Sep 14, 2010·International Journal of Biological Macromolecules·Hyunju LeeWan Jin Jahng
Jun 21, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Anne R DiersNeil Hogg
Apr 24, 2012·Biochimica Et Biophysica Acta·Kate Petersen ShayTory M Hagen
Dec 13, 2012·Science Signaling·Sahana Suresh BabuMarco Cattaruzza
Oct 3, 2014·Cold Spring Harbor Protocols·David A Clayton, Gerald S Shadel
Mar 4, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jana WobstHeribert Schunkert

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Citations

Jan 10, 2018·Frontiers in Pharmacology·Patrícia Molz, Nadja Schröder
Jul 6, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Daniele TibulloVincenzo Bramanti
Dec 22, 2020·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Chiara Di TucciPierluigi Benedetti Panici
Dec 29, 2020·The International Journal of Biochemistry & Cell Biology·Ajanta Chatterji, Rajib Sengupta
Apr 19, 2021·Biological Trace Element Research·Farzan MozaffarianMasoud Mahdavinia
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Jiabin ShiJianchun Bian

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