Alpha-macroglobulin secreted by alveolar macrophages serves as a binding protein for a macrophage-derived homologue of platelet-derived growth factor.

American Journal of Respiratory Cell and Molecular Biology
James C BonnerA R Brody

Abstract

Evidence is presented to support our hypothesis that an alpha-macroglobulin (alpha M) produced by lung macrophages serves as a specific binding protein for platelet-derived growth factor (PDGF) from these same macrophages. Culture medium "conditioned" by alveolar macrophages was fractionated by gel filtration according to molecular weight. Proteins larger than 200 kD were bound to greater than 50% of the macrophage-derived PDGF (MD-PDGF) that was extractable by 1 M acetic acid. Another approximately 25% was bound to fractions at approximately 150 kD, and approximately 20% remained unbound. The two high molecular weight fractions inhibited approximately 40% of specific [125I]PDGF binding to rat lung fibroblasts, whereas other fractions did not block PDGF binding to its receptor. Only the greater than 200 kD fractions inhibited the binding of PDGF antisera to purified human PDGF by 20% of control and exhibited specific complex formation and coelution on a gel filtration column with [125I]PDGF. The macrophage-derived alpha M (MD-alpha M) was separated from other macrophage-derived proteins by nickel-affinity chromatography and exhibited clear characteristics of alpha Ms, i.e., cross-reactivity with antibodies to human alpha 2-macr...Continue Reading

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Citations

Dec 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J B MangumA R Brody
Jun 23, 2004·Cytokine & Growth Factor Reviews·James C Bonner
Mar 1, 1991·Chest·A R Brody, J C Bonner
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Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·A SahniC W Francis
Jul 16, 2008·Journal of Clinical Laboratory Analysis·Yuhsaku KanohTohru Akahoshi
Aug 1, 1991·American Journal of Respiratory Cell and Molecular Biology·A Panyutich, T Ganz
Dec 1, 1990·American Journal of Respiratory Cell and Molecular Biology·A R Osornio-VargasA R Brody
Dec 1, 1991·American Journal of Respiratory Cell and Molecular Biology·W A MarinelliP B Bitterman
Nov 11, 2018·Particle and Fibre Toxicology·Alaeddin Bashir AbukabdaTimothy Robert Nurkiewicz
May 1, 1991·American Journal of Respiratory Cell and Molecular Biology·V G Kalter, A R Brody

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