Apr 5, 2020

Alpha-Smooth Muscle Actin-Positive Perivascular Cells in Diabetic Retina and Choroid

International Journal of Molecular Sciences
Soo Jin KimJunyeop Lee

Abstract

Structural alterations of pericytes in microvessels are important features of diabetic retinopathy. Although capillary pericytes had been known not to have α-smooth muscle actin (αSMA), a recent study revealed that a specific fixation method enabled the visualization of αSMA along retinal capillaries. In this study, we applied snap-fixation in wild type and streptozotocin-induced diabetic mice to evaluate the differences in vascular smooth muscle cells of the retina and the choroid. Mice eyeballs were fixed in ice-cold methanol to prevent the depolymerization of filamentous actin. Snap-fixated retina showed αSMA expression in higher-order branches along the capillaries as well as the arterioles and venules, which were not detected by paraformaldehyde fixation. In contrast, most choriocapillaris, except those close to the arterioles, were not covered with αSMA-positive perivascular mural cells. Large choroidal vessels were covered with more αSMA-positive cells in the snap-fixated eyes. Diabetes induced less coverage of αSMA-positive perivascular mural cells overall, but they reached higher-order branches of the retinal capillaries, which was prominent in the aged mice. More αSMA-positive pericytes were observed in the choroid of...Continue Reading

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Mentioned in this Paper

Protein Expression
Structure of Lamina Vasculosa of Choroid
Study
Pathogenesis
Fixation - Action
Quick Freeze
Myocytes, Smooth Muscle
Snap Brand of Resin
Detected (Finding)
Choroid Diseases

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