Alpha-tocopherol and BAY 11-7082 reduce vascular cell adhesion molecule in human aortic endothelial cells

Journal of Vascular Research
Ursula CatalánRosa Solà

Abstract

In endothelial dysfunction, vascular cell adhesion molecule-1 (VCAM-1), E-selectin and intercellular adhesion molecule-1 (ICAM-1) expression (collectively termed cell adhesion molecules; CAMs) increase at sites of atherosclerosis and are stimulated by proinflammatory cytokines such as tumor necrosis factor-α (TNF-α). We evaluated the effect of alpha-tocopherol (AT; 10-150 µM) and BAY 11-7082 (BAY; 0.1 or 1 µM) on CAMs mRNA expression as well as their protein in soluble release form (sCAMs) in human aortic endothelial cells (HAECs) activated by TNF-α (1 or 10 ng/ml). Also, we determined the extent of lymphocyte adhesion to activated HAECs. BAY reduced VCAM-1, E-selectin and ICAM-1 mRNA expression by 30, 30 and 10%, respectively. Furthermore, protein reduction of sVCAM-1 by 70%, sE-selectin by 51% and sICAM-1 by 25% compared to HAECs stimulated by TNF-α was observed (p < 0.05). AT (50, 75 and 150 µM) decreased VCAM-1 mRNA expression by 30% and sVCAM-1 protein by 33% compared to HAECs stimulated by TNF-α (p < 0.05). TNF-α-activated HAEC adhesion to human Jurkat T lymphocytes was higher compared to nonactivated HAECs (p < 0.05). BAY (2 and 5 µM) reduced this lymphocyte adhesion (p < 0.05). BAY reduces all the CAMs studied as well a...Continue Reading

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Citations

Apr 14, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yuan LiYan Chai
Jul 16, 2014·Pharmaceutical Biology·Yusof KamisahMohd Fahami Nur Azlina
Jun 3, 2015·Archives of Medical Research·Nimbe TorresArmando R Tovar
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May 29, 2015·Journal of Biomaterials Applications·Pilar FormentínLluís F Marsal
Oct 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Diego Estrada-LunaGabriel Betanzos-Cabrera

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