PMID: 9446836Mar 7, 1998Paper

Alpha-tocopherol in rat brain subcellular fractions is oxidized rapidly during incubations with low concentrations of peroxynitrite

The Journal of Nutrition
G T VatasseryHung T Quach

Abstract

The reaction of superoxide (a reactive oxygen species) and nitric oxide (one of the nitrogen oxides with numerous biological functions) results in the production of peroxynitrite. The characteristics of oxidation of alpha-tocopherol (vitamin E) in synaptosomes (nerve ending particles) and mitochondria by peroxynitrite were studied. The subcellular fractions were isolated from brain hemispheres of 4-month-old male Fischer 344 rats by standard centrifugation procedures involving Ficoll gradients. Peroxynitrite treatment oxidized alpha-tocopherol in <5 s. This reaction was selective because another membrane component, cholesterol, was not oxidized at the same time, as observed in our previous studies. Mitochondrial alpha-tocopherol was more susceptible to peroxynitrite-induced oxidation than synaptosomal tocopherol. Measurable and significant (P < 0.05) oxidation of tocopherol occurred when mitochondria or synaptosomes were incubated with peroxynitrite in concentrations as low as 5 or 10 micromol/L, respectively. The oxidation could be readily monitored by estimating the production of tocopherolquinone. Oxidation of tocopherol induced by ferrous iron and ascorbate was much slower and the yield of tocopherolquinone lower than by pe...Continue Reading

References

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Citations

Oct 11, 2007·Redox Report : Communications in Free Radical Research·Etsuo Niki
Jan 22, 2005·Clinical and Experimental Pharmacology & Physiology·M Kadkhodaee, A Qasemi
Jun 1, 2000·The Journal of Biological Chemistry·S ZimmerA Azzi
Aug 25, 2005·European Journal of Clinical Nutrition·M E van MeeterenE A F van Tol
May 4, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Daniela RibeiroEduarda Fernandes

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