Alpha-tocopheryl acetate is absorbed and hydrolyzed by Caco-2 cells comparative studies with alpha-tocopherol

Chemistry and Physics of Lipids
Lydie BrissonEl Hassan Ajandouz

Abstract

Caco-2 cells were used as a model for investigating and comparing the absorption of alpha-tocopherol (Tol) and alpha-tocopheryl acetate (Tac) solubilized in micelles based on a mixture of sodium taurocholate (NaTC) and oleic acid. Surprisingly, the uptake of Tac was found to be similar to that of Tol, and in both cases, the dose-response plots suggest that protein-mediated transport processes were involved. Moreover Tol or Tac were also secreted into the basolateral medium of Caco-2 cells but Tac was mainly hydrolyzed either prior to absorption or intracellularly. The solubilization of Tol or Tac by NaTC on the apical side of the cell monolayer is a prerequisite for the uptake process, although larger amounts of the bile salt are necessary to solubilize Tac than Tol. Caco-2 cells showed hydrolytic activity on Tac, and additional cholesterol esterase may be taken up by the cells, thus increasing the rates of intracellular hydrolysis of Tac. Based on our findings, a scheme is suggested accounting for the absorption of alpha-tocopheryl acetate by enterocytes.

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Citations

Jun 5, 2013·Nutrition Reviews·Patrick BorelCharles Desmarchelier
May 13, 2010·Journal of Microencapsulation·A BettencourtA J Almeida
Sep 2, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Jingyun WangWenbo Hao
Jun 19, 2013·Journal of Colloid and Interface Science·Ying Yang, David Julian McClements
Aug 24, 2010·Free Radical Biology & Medicine·Katarina JohanssonRalf Morgenstern
Aug 8, 2015·Journal of Oleo Science·Shiro KombaSachiko Machida
Feb 20, 2010·Molecular Nutrition & Food Research·Jean-Marc ZinggAngelo Azzi
May 3, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·A V NunesC M M Duarte
Mar 10, 2015·Archives of Dermatological Research·Ja Hyun RyuYong Chool Boo
Mar 9, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Bilal AbuasalAmal Kaddoumi
Jan 3, 2020·Drug Development and Industrial Pharmacy·Farzaneh NasiriMehrdad Hamidi

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