PMID: 8978820Dec 1, 1996Paper

alpha-Tubulin limits its own synthesis: evidence for a mechanism involving translational repression

The Journal of Cell Biology
M L Gonzalez-Garay, Fernando Cabral

Abstract

A Chinese hamster alpha-tubulin cDNA was modified to encode an 11-amino acid carboxyl-terminal extension containing the immunodominant epitope from influenza hemagglutinin antigen (to create HA alpha 1-tubulin) and was cloned into a vector for expression in mammalian cells. 12 stable CHO cell lines expressing this HA alpha 1-tubulin were isolated and characterized. HA alpha 1-tubulin incorporated into all classes of microtubules, assembled to the same extent as the endogenous tubulin, and did not perturb the growth of the cells in which it was expressed. However, overexpression of HA alpha 1-tubulin strongly repressed the synthesis of endogenous alpha-tubulin while having little or no effect on the synthesis of beta-tubulin. Treatment of transfected cells with sodium butyrate to induce even greater expression of HA alpha 1-tubulin led to a further decrease in synthesis of endogenous alpha-tubulin that was fully reversible upon removal of the inducer. Decreased synthesis of alpha-tubulin in transfected cells did not result from decreased levels of alpha-tubulin mRNA, as demonstrated by ribonuclease protection assays. On the other hand, colchicine, a drug previously shown to destabilize the tubulin message, caused a clear reducti...Continue Reading

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Citations

Jul 13, 1999·Cell Motility and the Cytoskeleton·M L FanarragaJ C Zabala
Jan 9, 1999·The Plant Journal : for Cell and Molecular Biology·R G Anthony, P J Hussey
Jun 26, 2009·The Journal of Biological Chemistry·Rebecca J Clifford, Jack H Kaplan
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Jul 4, 2007·The Plant Journal : for Cell and Molecular Biology·Antanas V SpokeviciusGerd Bossinger
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Aug 14, 1999·The Journal of Biological Chemistry·M L Gonzalez-GarayF Cabral

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