Alpha-V-dependent outside-in signaling is required for the regulation of CD44 surface expression, MMP-2 secretion, and cell migration by osteopontin in human melanoma cells

Experimental Cell Research
V SamannaMeenakshi A Chellaiah

Abstract

The level of integrin alpha(v)beta3 and its ligand osteopontin (OPN) has been directly correlated to tumorigenicity of melanoma and other cancer cells. We have previously shown an increase in pp(60c-Src) kinase activity associated with integrin alpha(v)beta3 in melanoma cells (M21) treated with soluble OPN. pp(60c-Src) kinase activity was not observed in melanoma cells expressing alpha(v) that lacks the cytoplasmic domain (alpha(v)995). Results of the current study demonstrate that the amino acid sequence '995RPPQEEQERE1004' in the beta-turn of alpha(v) chain is required for the interaction of pp(60c-Src). Our results suggest that the beta-turn of alpha(v) chain may be indispensable for alpha(v)-associated signaling complex formation and outside-in signaling. To further analyze the alpha(v)beta3 signaling in melanoma cells, we over expressed OPN in M21 cells (M21/OPN). CD44 surface expression and MMP-2 activity in the conditioned medium were increased to a greater extent in M21/OPN cells as compared with M21 or alpha(v)995 cells. Also, M21/OPN cells exhibit increased motility, which is markedly reduced upon treatment with inhibitors to alpha(v) and MMP-2. Our findings suggest that the increase in MMP-2 activity is integrin-depe...Continue Reading

References

Mar 1, 1992·Clinical & Experimental Metastasis·K R GehlsenP Sriramarao
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Jun 1, 1992·The Journal of Clinical Investigation·B Felding-HabermannD A Cheresh
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F P ReinholtD Heinegård
Jun 1, 1991·Cancer Metastasis Reviews·I R HartJ F Marshall
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Aug 1, 1990·The Journal of Cell Biology·C A OteyK Burridge
Apr 25, 1989·Nucleic Acids Research·M C KieferP J Barr
Jan 1, 1995·Methods in Enzymology·K TakaishiY Takai
Sep 1, 1995·The Journal of Cell Biology·M D SchallerJ T Parsons
Jul 1, 1993·The Journal of Cell Biology·J S BauerR L Juliano
Jan 1, 1997·Advances in Cancer Research·D NaorD Ish-Shalom
Aug 1, 1997·Glycoconjugate Journal·J LesleyG A Turner
Jun 13, 1998·The Journal of Biological Chemistry·M ChellaiahK Hruska
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·K TakahashiK K Tanabe
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Feb 23, 2000·The Journal of Cell Biology·M ChellaiahK A Hruska
Apr 15, 2000·The Journal of Biological Chemistry·M A ChellaiahK A Hruska
Aug 22, 2000·The Journal of Investigative Dermatology·U B HofmannD J Ruiter
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M WozniakK A Hruska
Oct 6, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J SodekM D McKee
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·D T DenhardtS R Rittling
Apr 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H NemotoM Noda
Feb 5, 2002·Biochimica Et Biophysica Acta·G F Weber
Mar 21, 2002·Cell and Tissue Research·Jane L FisherGail P Risbridger
Sep 18, 2002·The Journal of Cell Biology·Paola SpessottoValter Gattei
Dec 7, 2002·Calcified Tissue International·M A Chellaiah, K A Hruska
Dec 21, 2002·Differentiation; Research in Biological Diversity·Mei-Yu HsuMeenhard Herlyn
Jan 17, 2003·Molecular Biology of the Cell·M A ChellaiahK A Hruska
May 6, 2003·The Journal of Biological Chemistry·Meenakshi A ChellaiahKeith A Hruska

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Citations

Oct 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John A ContiJohn P Iredale
Jul 24, 2010·The Journal of Biological Chemistry·Tao MaMeenakshi A Chellaiah
Sep 2, 2010·PloS One·Regina Berretta, Pablo Moscato
Sep 24, 2011·Journal of Oncology·Stephan Niland, Johannes A Eble
Nov 24, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Ryan N Serio
Nov 12, 2013·Cell Communication & Adhesion·Samarth Hegde, Srikala Raghavan
May 4, 2016·Current Stem Cell Reports·Efraín A Cermeño, Andrés J García
Mar 29, 2011·Trends in Cell Biology·Sara A Wickström, Reinhard Fässler
Nov 10, 2009·Ageing Research Reviews·Mitchell KamravaElise Kohn
Dec 25, 2007·Biochemical and Biophysical Research Communications·Leah Forquer RahejaClare E Yellowley
Apr 7, 2007·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·David R ClemmonsWalker H Busby
Mar 12, 2013·TheScientificWorldJournal·Aditi GuptaMeenakshi A Chellaiah
Oct 3, 2007·Journal of Cellular Biochemistry·Christian C KazaneckiDavid T Denhardt
May 18, 2007·Journal of Cellular Physiology·V SamannaM A Chellaiah
Mar 25, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Lalita A Shevde, Rajeev S Samant
Oct 10, 2014·Surgery Today·Daisuke IzumiHideo Baba
May 13, 2009·Melanoma Research·Jade HomsiAdil I Daud
Dec 24, 2009·Nature Reviews. Cancer·Jay S Desgrosellier, David A Cheresh
Mar 23, 2017·Frontiers in Cell and Developmental Biology·Linda T Senbanjo, Meenakshi A Chellaiah
Jun 1, 2018·Scientific Reports·Steffen VoglerGerd Kempermann
May 15, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adil AnwarKurt R Stenmark
Jan 29, 2021·Stem Cells International·Priscila Anhel Medrano-GonzálezErika P Rendón-Huerta
Mar 9, 2007·Molecular Cancer·Bhavik DesaiMeenakshi A Chellaiah

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