Alpha1-adrenergic receptor signaling via Gh is subtype specific and independent of its transglutaminase activity.

The Journal of Biological Chemistry
S ChenRobert M Graham

Abstract

Tissue transglutaminase (TGase II) is a Ca2+- and thiol-dependent enzyme that catalyzes the post-translational modification of proteins via the formation of epsilon(gamma-glutamyl) lysine bonds. We have shown previously that the GTP-binding protein, Gh, is a TGase II that mediates intracellular signaling by the alpha1B-adrenergic receptor (AR) (Nakaoka, H., Perez, D. M., Baek, K. J., Das, T., Husain, A., Mison, K., Im, M.-J., and Graham, R. M. (1994) Science 264, 1593-1596). Here, we evaluated the ability of Gh as compared with Gq to mediate receptor-stimulated inositol phosphate turnover by the three alpha1-subtypes (alpha1A, alpha1B, and alpha1D). In addition, we questioned if the transglutaminase function of Gh is involved in its receptor signaling activity. A mutant form of a human TGase II cDNA in which the codon for the active site cysteine (Cys277) was replaced by serine was cloned into the mammalian expression vector pMT2'. Compared with wild-type TGase II, no transglutaminase activity was observed with transient transfection of this Cys-->Ser mutant in COS-1 cells. However, like wild-type TGase, the Cys-->Ser mutant mediated receptor-stimulated inositol phosphate turnover when cotransfected with an alpha1B-AR cDNA. Gal...Continue Reading

References

Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Jan 1, 1990·Methods in Enzymology·R J Kaufman
Jan 1, 1985·Hybridoma·P J BirckbichlerE Conway
Jan 1, 1980·Annual Review of Biochemistry·J E Folk
Oct 1, 1995·Journal of Molecular and Cellular Cardiology·E VincanE A Woodcock
May 1, 1996·Circulation Research·R M GrahamM T Piascik

❮ Previous
Next ❯

Citations

Aug 5, 2000·Cancer·P J BirckbichlerG P Hemstreet
Oct 21, 1999·Cell Biology International·E GiontiR Porta
Dec 8, 2010·Journal of Neural Transmission·D Ramakrishna, M N Subhash
Jun 23, 2004·Biology of the Cell·Sakina Mhaouty-Kodja
Mar 23, 2004·Molecular and Cellular Neurosciences·Craig D C BaileyGail V W Johnson
May 5, 2001·Pharmacology & Therapeutics·G A MichelottiD A Schwinn
Dec 22, 1999·Pharmacology & Therapeutics·C Saunders, L E Limbird
Dec 12, 2001·Neurochemistry International·Bruce A CitronBarry W Festoff
Apr 5, 2000·Progress in Neurobiology·M LesortG V Johnson
Mar 14, 1997·Molecular and Cellular Endocrinology·E A Woodcock
Dec 7, 2007·Biochemistry·Sunando DattaRichard A Cerione
Apr 1, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anthony J FilianoGail V W Johnson
Jun 24, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Christopher J ClarkeJacqueline Ohanian
May 30, 2013·PloS One·Siiri E IismaaRobert M Graham
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·David C SaneCharles S Greenberg
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Maria Christina W AvellarCatarina S Porto
Nov 30, 2005·Biochemical and Biophysical Research Communications·Suresh Mishra, Liam J Murphy
Dec 9, 2004·Journal of Neurochemistry·Craig D C Bailey, Gail V W Johnson
Jun 15, 2004·The Journal of Biological Chemistry·Zhongjian ChenKenneth P Minneman
May 5, 2007·The Journal of Biological Chemistry·Suresh MishraLiam J Murphy
Feb 14, 2004·The Journal of Biological Chemistry·Songhai ChenHeidi E Hamm
Feb 19, 2004·The Journal of Biological Chemistry·Morgan DupuisSakina Mhaouty-Kodja
Apr 19, 2003·Circulation Research·Zhibing ZhangGarret A FitzGerald
Oct 29, 2004·The Journal of Biological Chemistry·Yuanjian GuoSuzanne Scarlata
May 29, 2008·The Journal of Physiological Sciences : JPS·A W Lee, D W Pfaff
Mar 15, 2006·The Journal of Biological Chemistry·Zhongjian ChenKenneth P Minneman
Apr 3, 2014·Physiological Reviews·Richard L EckertKapil Mehta
May 25, 2004·Molecular Pharmacology·Dezhong YinCraig C Malbon
May 4, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Salvatore CondelloDaniela Matei

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.