Alpha1-adrenoceptor subtype activation increases proto-oncogene mRNA levels. Role of protein kinase C

European Journal of Pharmacology
J A García-SáinzJ Vázquez-Prado

Abstract

Noradrenaline increased the mRNA levels of c-fos and c-jun in rat-1 fibroblast lines stably expressing the cloned alpha1-adrenoceptor subtypes. The efficacy to induce the expression of c-fos mRNA was similar for the three cell lines (alpha1d = alpha1b = alpha1a) but different for c-jun (alpha1a > or = alpha1b > alpha1d). The EC50 values were also different: approximately 5 nM (c-fos) and approximately 300 nM (c-jun) for cells transfected with the alpha1a subtype, approximately 30 nM (c-fos) and approximately 300 nM (c-jun) for cells transfected with the alpha1b subtype and approximately 300 nM (c-fos and c-jun) for those transfected with the alpha1d subtype. Staurosporine and protein kinase C down-regulation blocked such effects, indicating a role of this protein kinase. Endothelin-1 (10 nM) also increased the levels of c-fos and c-jun mRNAs. These actions of endothelin-1 were unaffected by staurosporine and protein kinase C down-regulation. It is concluded that activation of any of the three cloned subtypes can increase the levels of c-fos and c-jun mRNAs and that protein kinase C plays a major role in mediating such effects.

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Citations

Jul 16, 2010·Reproductive Biology and Endocrinology : RB&E·Francisco G Vázquez-CuevasRogelio O Arellano
Oct 5, 2007·Burns : Journal of the International Society for Burn Injuries·Yasuhiro TsudaFujio Suzuki
Oct 7, 2004·European Journal of Pharmacology·J Adolfo García-Sáinz, Rafael Villalobos-Molina
Feb 29, 2000·The Journal of Biological Chemistry·J Vázquez-PradoJ A García-Sáinz
Mar 24, 2009·Autonomic & Autacoid Pharmacology·R Alcántara-Hernández, J Adolfo García-Sáinz
Feb 20, 2009·Molecular and Cellular Biochemistry·Tushi SingalParamjit S Tappia
Dec 23, 1999·European Journal of Pharmacology·J A García-SáinzM T Romero-Avila
Dec 22, 1999·Archives of Medical Research·J A García-SáinzJ Vázquez-Prado
Mar 14, 2000·Archives of Medical Research·J A García-SáinzR Villalobos-Molina

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